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  5. Invitrogen™ Human EN-RAGE/S100A12 ELISA Kit

Invitrogen™ Human EN-RAGE/S100A12 ELISA Kit

Product Code. 17184919
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Quantity:
10 x 96 Tests
5 x 96 Tests
96 Tests
Unit Size:
Each
Pack of 10
Pack of 96
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Product Code. 17184919

Brand: Invitrogen™ EH170RBX5

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Description

ELISA

Human EN-RAGE/S100A12 quantitates human EN-RAGE/S100A12 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human EN-RAGE/S100A12.

S100A12 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A12 is proposed to be involved in specific calcium-dependent signal transduction pathways and its regulatory effect on cytoskeletal components may modulate various neutrophil activities. S100A12 is also a ligand for RAGE. Interaction with RAGE on mononuclear phagocytes, lymphocytes and endothelium triggers cellular activation. Nuclear factor kappa B, a key transcription factor for inflammatory events, is activated by S100A12/RAGE. S100A12 has also been shown to have anti-microbial properties.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number CAAF1; CAGC; Calcitermin; calcium-binding protein in amniotic fluid 1; calgranulin C; calgranulin-C; CGRP; EN RAGE; ENRAGE; EN-RAGE; Extracellular newly identified RAGE-binding protein; Migration inhibitory factor-related protein 6; MRP6; MRP-6; Neutrophil S100 protein; p6; Protein S100 A12; Protein S100-A12; S100 calcium binding protein A12; S100 calcium-binding protein A12; S100 calcium-binding protein A12 (calgranulin C); S100A12
Assay Range 0.82 to 200 ng/mL
Assay Sensitivity 0.82 ng/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 6283
Gene Symbol S100A12
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 5 x 96 Tests
Regulatory Status RUO
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Storage Requirements 2°C to 8°C
Target S100A12
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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