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Gibco™ Human Dkk-1 Recombinant Protein

Product Code. 10023534
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Quantity:
1 mg
10 μg
100 μg
25 μg
Unit Size:
Each
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Product Code. 10023534

Brand: Gibco™ PHC9214

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Description

Recombinant Protein

Carrier-Free Reconstitution: Centrifuge the vial briefly, before opening to bring the contents to the bottom. Reconstitute the lyophilized protein in sterile, distilled water to a concentration of 0.1-0.5 mg/mL. Apportion the reconstituted protein into working aliquots and store at ≤-20°C. Make any further dilutions of the reconstituted protein in low endotoxin medium or a buffered solution containing a carrier protein such as heat inactivated FCS or tissue culture grade BSA. Storage: Store the lyophilized protein at 2-8°C or -20°C long term, preferably desiccated. Upon reconstitution, apportion into working aliquots and store at ≤-20°C. Avoid repeated freeze-thaw cycles.

DKK-1 is a member of the DKK protein family which also includes DKK-2, DKK-3 and DKK-4. DKK-1 was originally identified as a Xenopus head-forming molecule that behaves as an antagonist for Wnt signaling. Subsequent studies have shown that DKK-1 and DKK-4 play important regulatory roles in the Wnt/beta-catenin signaling pathway by forming inhibitory complexes with LDL receptor-related proteins 5 and 6 (LRP5 and LRP6), which are essential components of the Wnt/beta-catenin signaling system. LRP5 and LRP6 are single-pass transmembrane proteins that appear to act as co-receptors for Wnt ligands involved in the Wnt/beta-catenin signaling cascade. It has been suggested that by inhibiting Wnt/beta-catenin signaling, which is essential for posterior patterning in vertebrates, DKK-1 permits anterior development. This notion is supported by the finding that mice deficient of DKK-1 expression lack head formation and die during embryogenesis.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number O94907
For Use With (Application) Bioactivity
Formulation Protein with no preservative
Gene ID (Entrez) 22943
Molecular Weight (g/mol) 42 kDa
Name Human Dkk-1
Purification Method Purified
Quantity 10 μg
Storage Requirements 4°C
Regulatory Status RUO
Endotoxin Concentration <0.1 ng/μg
Gene Alias dickkopf 1 homolog; dickkopf homolog 1; dickkopf homolog 1 (Xenopus laevis); dickkopf related protein-1; dickkopf WNT signaling pathway inhibitor 1; Dickkopf1; dickkopf-1; dickkopf-1 like; dickkopf-like protein 1; dickkopf-related protein 1; DKK1; DKK-1; hDkk1; hDkk-1; mdkk-1; SK; UNQ492/PRO1008
Common Name DKK1
Gene Symbol DKK1
Biological Activity ED50 ≤ 40 ng/mL; determined by the binding of immobilized human LRP6/Fc chimera receptro (3 μg/mL) to human Dkk-1 in. a functional ELISA.
Product Type Protein
Conjugate Unconjugated
Species Human
Recombinant Recombinant
Content And Storage 4°C
Classification Carrier-Free
Activity ED50 ≤ 40 ng/mL; determined by the binding of immobilized human LRP6/Fc chimera receptro (3 μg/mL) to human Dkk-1 in a functional ELISA.
Endotoxin Level <0.1 ng/μg
Reactivity Human
Shipping Condition Wet Ice
Specific Activity Linear range of 0.3 to 40 ng⁄ml
Expression System HEK293 cells
Protein Family Cytokines & Receptors
Protein Form Recombinant, Ligand
Form Lyophilized
Protein Subtype Other Proteins
Research Category Oncology, Signal Transduction
Product Line Gibco
Purity or Quality Grade ≥95% by SDS-PAGE and HPLC
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