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Gibco™ Human BDNF Recombinant Protein

Product Code. 10477253
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Quantity:
10 μg
Unit Size:
Each
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Product Code. 10477253

Brand: Gibco™ PHC7074

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Description

Recombinant Protein

Carrier-Free Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute BDNF in sterile, distilled water to a concentration of 0.1-1.0 mg/mL. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing carrier protein, such as 0.1% BSA and store in working aliquots from -20°C to -80°C. Storage: The lyophilized protein is stable at room temperature for up to 1 month, but should be kept at -20°C for long term storage. Upon reconstitution, the preparation is stable for up to one week at 2°C to 8°C. Working aliquots stored with a carrier protein are stable for at least 12 months at -20°C to -80°C. Avoid repeated freeze-thaw cycles.

BDNF (Brain-Derived Neurotrophic Factor) is a member of the neurotrophin family. BDNF is synthesized as pre-proBDNF, followed by cleavage to proBDNF. Although further processing generates the mature, 14 kDa protein, pro-BDNF is biologically active and is secreted in synaptic vesicles along with the mature form. BDNF is widely expressed in the central nervous system and acts in an autocrine and paracrine manner on several classes of neurons. Signaling occurs mainly through the tyrosine kinase receptor TrkB, although binding to the lower-affinity receptor, p75-NTR, has also been demonstrated. BDNF promotes neuronal survival and differentiation and has been shown to play a critical role in memory formation and synaptic regulation. BDNF is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of BDNF is reduced in both Alzheimer's and Huntington disease patients, and may also play a role in the regulation of stress response and in the biology of mood disorders.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P23560
For Use With (Application) Bioactivity
Formulation Protein with no preservative
Gene ID (Entrez) 627
Molecular Weight (g/mol) 27 kDa
Name Human BDNF
Purification Method Purified
Quantity 10 μg
Storage Requirements -20°C
Regulatory Status RUO
Endotoxin Concentration <0.1 ng/μg
Gene Alias abrineurin; ANON2; anorexia BDNF; Bdnf; BDNF precursor form; bdnf protein; brain derived neurothrophic factor; brain derived neurotrophic factor; brain-derived neurotrophic factor; BULN2; H-BDNF; neurotrophin; ProBDNF
Common Name BDNF
Gene Symbol BDNF
Biological Activity ED50 = 0.5 - 1.0 μg/mL; determined by the dose-dependent proliferation of rat C6 cells.
Product Type Protein
Conjugate Unconjugated
Species Human
Recombinant Recombinant
Sequence Human BDNF recombinant protein is a homodimer containing two 120 amino acid subunits linked by hydrophobic interaction
Content And Storage -20°C
Classification Carrier-Free
Activity ED50 = 0.5 - 1.0 μg/mL; determined by the dose-dependent proliferation of rat C6 cells.
Endotoxin Level <0.1 ng/μg
Shipping Condition Approved for shipment on Wet or Dry Ice
Expression System E. coli
Protein Family Growth Factors & Receptors
Protein Form Recombinant, Ligand
Form Lyophilized
Protein Subtype Neurotrophic Factors
Research Category Neurobiology, Stem Cell Research, Differentiation
Product Line Gibco
Purity or Quality Grade ≥98% by SDS-PAGE and HPLC
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