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  5. Invitrogen™ Granzyme B Human ELISA Kit

Invitrogen™ Granzyme B Human ELISA Kit

Product Code. 15551517
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10 x 96 Tests
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Pack of 10
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15551517 10 x 96 Tests Pack of 10
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Product Code. 15551517 Supplier Invitrogen™ Supplier No. BMS2027TEN

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Description

Includes

  • Aluminum pouch(es) with a Microwell Plate coated with monoclonal antibody to human Granzyme B
  • Biotin-Conjugate anti-human Granzyme B monoclonal antibody
  • Streptavidin-HRP
  • Human Granzyme B Standard lyophilized, 960pg/mL upon reconstitution
  • Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
  • Dilution Buffer Concentrate 5x
  • Substrate Solution (tetramethyl-benzidine)
  • Stop Solution (1M Phosphoric acid)
  • Blue-Dye
  • Green-Dye
  • Red-Dye
  • Adhesive Films

For the quantitative detection of human Granzyme B.

The Human Granzyme B ELISA quantitates Hu Granzyme B in human serum or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Granzyme B. Principle of the method The Human Granzyme B solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Granzyme B is a member of the granzyme serine protease family, and is found in the granules of cytotoxic T cells and NK cells. Granzyme B has been described as CGL1 (cathepsin G-like-1), a serine protease expressed only in cytotoxic T-lymphocytes after cell activation, and CTLA-1 (cytotoxic T lymphocyte-associated serine esterase 1) based on identification of mRNA in various cytotoxic T cells, but not observed in non-cytotoxic lymphoid cells. Granzyme B is crucial for the rapid induction of target cell death by apoptosis, induced by interaction with cytotoxic T cells. The receptor involved in this process has been identified as mannose 6-phosphate receptor which functions as a death receptor for Granzyme B during cytotoxic T cell-induced apoptosis. Granzyme B enters target cells to cleave caspase-3 and initiate the caspase cascade leading to DNA fragmentation and apoptosis. Granzyme B can also act through a mitochondrial apoptosis pathway by cleaving the Bid protein. Granzymes are neutral serine proteases, which are stored in specialized lytic granules of cytotoxic T lymphocytes (CTLs) and in natural killer (NK) cells. A number of granzymes (A to G) have been isolated and cloned from mouse CTLs and NK cells, however in man, fewer have been cloned and identified.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P10144
Assay Range 0.66-480 pg/mL
Assay Sensitivity 0.2 pg/mL
Conjugate Biotin
Product Type ELISA Kit
Sample Type Serum, Supernatant
Target Species Human
For Use With (Application) ELISA
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 3002
Gene Symbol C11,CCPI,CGL-1,CGL1,CSP-B,CSPB,CTLA1,CTSGL1,HLP,SECT
Interassay CV 10.4%
Intraassay CV 8.5%
Kit Contents Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 10 x 96 Tests
Regulatory Status RUO
Sample Volume Serum, 50 μL; Supernatant, 50 μL
Storage Requirements 2°C to 8°C
Synonym Granzyme 2, Fragmentin 2, Human lymphocyte protein
Target Human
Test Time 1 hr. 20 min.
Total Assay Time 2 hr. 45 min.
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For Research Use Only

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