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Gibco™ Advanced RPMI 1640 Medium

Advanced RPMI (Roswell Park Memorial Institute) 1640 is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation.

36.48 EUR - 326.00 EUR

Specifications

Content And Storage Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Cell Line Sp2, Vero, Raji, Daudi, and Jurkat
Classification Animal Origin
Concentration 1 X
Form Liquid
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Product Code Brand Quantity Price Quantity & Availability  
11520446
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Gibco™
12633012
500 mL
36.48 EUR
500mL
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11530446
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Gibco™
12633020
10 x 500 mL
326.00 EUR
Pack of 10
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Description

Description

Advanced RPMI (Roswell Park Memorial Institute) 1640 is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic RPMI 1640, serum supplementation can be reduced by 50-90% with no change in growth rate or morphology.i Cells successfully cultured in Advanced RPMI 1640, with no adaptation, include Sp2, Vero, Raji, Daudi, and Jurkat.

This Advanced RPMI 1640 is manufactured as follows:
With: Glucose, Non-essential Amino Acids, Sodium Pyruvate, Phenol Red
Without: L-glutamine, HEPES

The complete formulation is available.

Gibco™ Advanced RPMI 1640 is unique from other media due to addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX™ I lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride.

Product Use
For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.

Advanced RPMI 1640 requires supplementation with 1-5% Fetal Bovine Serum and 4 mM L-glutamine or GlutaMAX™ supplement. Many cell lines do not require adaptation to this media. The FBS concentration must be optimized for each cell line to obtain maximum serum reduction. Advanced RPMI 1640 uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

iAdvanced Media: Reduced Sera Solutions. Quest, 2004. 1.3:26-30.

Order Info

Shipping Condition: Room Temperature

Compliance

Gibco Advanced RPMI 1640 is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.

Specifications

Specifications

Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Animal Origin
Liquid
Reduced Serum
Glucose, Phenol Red, Sodium Pyruvate, Non Essential Amino Acids (NEAA)
Sustainable packaging
Gibco™
Sp2, Vero, Raji, Daudi, and Jurkat
1 X
RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)
Sterile-filtered
No Glutamine, No HEPES
cGMP-compliant under the ISO 13485 standard
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Product Certifications

For Research Use Only. Not for use in diagnostic procedures.