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  6. Molecular Probes™ Fluo-4 Direct™ Calcium Assay Kit

Molecular Probes™ Fluo-4 Direct™ Calcium Assay Kit

Product Code. 10297783
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Quantity:
10 x 10 mL
1 x 100 mL
1 x 1 L
Unit Size:
Each
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Product Code. 10297783

Brand: Molecular Probes™ F10471

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This item is not returnable. View return policy

Description

Fluo-4 Direct™ Calcium Assay Kit

The Fluo-4 Direct™ Calcium Assay Kit was formulated to provide a homogeneous fluorescent calcium assay that:
1. Easily loads into cells
2. Achieves a large assay window and
3. Suppresses background fluorescence generated from the calcium indicator in complete media with little-to-no impact on the specific cellular fluorescence

This advanced formulation allows the assay to be run in a simple addition only format in the presence of serum-containing media. Fluo-4 Direct™ is the third addition to the Fluo-4 family of calcium detection reagents. Fluo-4 AM and Fluo-4 NW both require media removal before assay detection, but Fluo-4 NW adds convenience by including the PowerLoad™ reagent for ease in cell loading. The Fluo-4 Direct™ Calcium Assay Kit is similar to Fluo-4 NW in that it is formulated with PowerLoad™ for ease in cell loading, but unique in that it can be used in the presence of complete culture media and will efficiently suppress background fluorescence without sacrificing the specific cellular fluorescence generated in the assay.
TRUSTED_SUSTAINABILITY

Specifications

Color Green
Content And Storage
  • 10 × 10 ml Fluo-4 Direct™ Calcium Assay Reagent (Component A)
  • 2 × 77 mg Probenecid (Component B)
  • 200 ml Fluo-4 Direct™ Calcium Assay Buffer (Component C)
  • Store at ≤-20°C. Dessicate and protect from light.
Detection Method Fluorescence
For Use With (Application) Calcium Assay
For Use With (Equipment) Confocal Microscope, Fluorescence Microscope, High Content Analysis Instrument, HTS Reader, Microplate Reader, Fluorescent Imager
Product Type Calcium Assay Kit
Dye Type Fluorescent Dye-Based
Emission Visible
Form Powder
Modification Chemical
Product Line Molecular Probes
Quantity 10 x 10 mL
Shipping Condition Room Temperature
Sub Cellular Localization Nucleus, Organelles, Cytoplasm
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For Research Use Only. Not for use in diagnostic procedures.

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