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  5. Invitrogen™ Human FGF4 (Cell Lysates) ELISA Kit

Invitrogen™ Human FGF4 (Cell Lysates) ELISA Kit

Product Code. 15434993
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Quantity:
5 x 96 Tests
96 Tests
Unit Size:
1 set
Each
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15434993 96 Tests 1 set
17165439 5 x 96 Tests Each
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Product Code. 15434993 Supplier Invitrogen™ Supplier No. EHFGF4CL

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Description

ELISA

The Human Fibroblast Growth Factor 4 Cell Lysates (Hu FGF4CL) ELISA quantitates Hu FGF4CL in human cell and tissue lysates. The assay will exclusively recognize both natural and recombinant Hu FGF4CL. Principle of the method The Human FGF4CL solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Fibroblast growth factor 4 (FGF4) is a member of the fibroblast growth factor (FGF) family that possess broad mitogenic and cell survival activities and play key roles in growth and survival of stem cells during embryogenesis, tissue regeneration, and carcinogenesis. FGF4 was identified by its strong oncogenic transforming activity and is a potent angiogenic factor, expressed in several highly vascularized tumors and also in adult mouse testis, intestine, and brain. Studies on the mouse homolog suggests a function in bone morphogenesis and limb development through the sonic hedgehog (SHH) signaling pathway. Furthermore, FGF4 regulates neural progenitor cell proliferation and neuronal differentiation. Recent studies show a growth-promoting role for FGF4 in human embryonic stem cells and a putative feedback inhibition mechanism by a novel FGF4 splice isoform that may serve to promote differentiation at a later stages of development.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P08620
Assay Range 24.69 to 18,000 pg/mL
Assay Sensitivity 50 pg/mL
Calibration Range 50 pg/mL to 18000 pg/mL
Assay Solid-phase sandwich ELISA
Conjugate HRP
Product Type ELISA
Protein Family Growth Factors & Receptors
Sample Type Cell Lysates
Specificity The antibody pair provided in this kit recognizes human FGF-4.
Sufficient For 96 assays
Target Species Human
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 2249
Gene Symbol HBGF-4,HST,HST-1,HSTF1,K-FGF,KFGF
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Cell Lysis Buffer, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 96 Tests
Regulatory Status RUO
Research Discipline Apoptosis
Sample Volume Cell Lysate 20 μL
Storage Requirements 2°C to 8°C
Synonym HBGF-4, HST, HST-1, HSTF1, K-FGF, KFGF
Target FGF4
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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