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Maxima Maxima SYBR Green qPCR Master Mix (2X), with separate ROX vial
Ready-to-use solution optimized for qPCR and 2-step RT-qPCR.
Brand: Maxima K0252
998.62 EUR valid until 2024-12-31
Use promo code "21881" to get your promotional price.
Description
The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.
Maxima Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carry-over contamination control using uracil DNA glycosylase (UDG). The use of Maxima SYBR Green qPCR Master Mixes in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral, and cDNA templates. Maxima SYBR Green qPCR Master Mixes are compatible with most real-time thermal cyclers.
- Specificity - Maxima Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
- Sensitivity - detects low copy number targets
- Wide linear range - accurate quantification across 9 orders of magnitude
- Reproducibility and convenience - ready-to-use 2X master mix
Specifications
Contains: • 10 x 1.25 mL Maxima SYBR Green qPCR Master Mix (2X) containing Maxima Hot Start Taq DNA Polymerase, dNTPs (also dUTP) and SYBR Green I in an optimized PCR buffer; sufficient for 1000 x 25 μL reactions. • 250 μL ROX solution, 50 μM • 10 x 1.25 mL Nuclease-Free Water Store at -20°C. |
|
Tube | |
qPCR | |
Standard | |
Maxima | |
1000 Reactions at 25 μL | |
2X | |
DNA, dsDNA |
SYBR | |
High | |
Taq DNA Polymerase | |
BioRad CFX96™, MJ Chromo4, MJ Opticon, Eppendorf MasterCycler RealPlex, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, Corbett RotorGene | |
Real Time PCR SYBR Master Mix | |
Gene Expression | |
1,000 Reactions | |
10 x 1.25 mL |
For Research Use Only. Not for use in diagnostic procedures.