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  5. Thermo Scientific™ Biotin DecaLabel DNA Labeling Kit

Thermo Scientific™ Biotin DecaLabel DNA Labeling Kit

Product Code. p-4530754 Shop All Thermo Scientific Products
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30 reactions
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Product Code. No. of Reactions unitSize
10880231 10 10 reactions
10890231 30 30 reactions
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Product Code. 10880231 Supplier Thermo Scientific™ Supplier No. K0651

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Description

Efficiently synthesize biotin-labeled DNA probes with the Thermo Scientific™ Biotin DecaLabel DNA Labeling Kit.

Biotin DecaLabel DNA Labeling Kit is an advanced system for the efficient synthesis of biotin-labeled DNA probes, based on an improved random-primed labeling method developed by Feinberg and Vogelstein. The primary improvement over traditional random-primed method involves the use of random decamers instead of hexamers, ensuring more efficient annealing with DNA at 37°C. Klenow Fragment, exo-, included in the kit, is genetically engineered enzyme with no detectable exonuclease activity. The enzyme does not degrade the labeled probe during reaction, which results in a high labeling yield even with low amounts of template. You can uniformly label any length DNA fragments.

Biotin-labeled DNA is detected with the Biotin Chromogenic Detection Kit or conventional biotin-avidin or biotin-streptavidin detection systems.

The primary improvement over traditional random-primed method involves the use of random decamers instead of hexamers to ensure more efficient annealing with DNA at 37°C. Klenow Fragment, exo- is also included in the kit; this genetically engineered enzyme has no detectable exonuclease activity. Therefore, the enzyme does not degrade the labeled probe during reaction, which results in a high labeling yield even with low amounts of template. As a result, DNA fragments of any length can be uniformly labeled. Biotin-labeled DNA is detected with the Biotin Chromogenic Detection Kit or with conventional biotin-avidin or biotin-streptavidin detection systems.
  • Non-radioactive labeling of DNA
  • Efficient priming of labeling reactions with random decamers
  • High yields with Klenow Fragment, exo-: no degradation of a labeled probe during reaction

Recommended for:

Generation of biotin-labeled DNA probes for a variety of non-radioactive hybridization experiments, including Southern and Northern blots, colony/plaque hybridizations, dot/slot blots and in situ hybridizations

TRUSTED_SUSTAINABILITY

Specifications

Detection Method Biotin-based
Format Kit
Final Product Type DNA
Labeling Target Genomic DNA, PCR Products, DNA (General)
Includes Label or Dye Yes
Labeling Method Direct Labeling
No. of Reactions 10
Product Line DecaLabel
Product Type DNA Labeling Kit
Quantity 10 Reactions
Product Title
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