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  6. Invitrogen™ eBioscience™ Annexin V Apoptosis Detection Kits

Invitrogen™ eBioscience™ Annexin V Apoptosis Detection Kits

Product Code. 14819780
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Quantity:
100 Tests
20 Tests
200 Test
200 Tests
300 Tests
50 Tests
Conjugate:
APC, Propidium Iodide
Biotin, Propidium Iodide
FITC, Propidium Iodide
PCP-eFluor 710
PE, 7-AAD
PE/Cy7
eFluor 450, 7-AAD
Unit Size:
100 tests
20 tests
200 tests
300 tests
50 tests
Each
Excitation/Emission:
405, 546/450, 647
488, 535/520, 617
650, 535/660, 617
NA, 535/NA, 617
18 product options available for selection
Product selection table with 18 available options. Use arrow keys to navigate and Enter or Space to select.
Product Code. Quantity Conjugate unitSize Excitation/Emission
14819780 50 Tests PE, 7-AAD 50 tests -
12770170 200 Tests FITC, Propidium Iodide Each 488, 535/520, 617
15561187 50 Tests eFluor 450, 7-AAD 50 tests 405, 546/450, 647
15571187 200 Tests eFluor 450, 7-AAD 200 tests 405, 546/450, 647
12780170 200 Tests APC, Propidium Iodide Each 650, 535/660, 617
15591187 50 Tests PCP-eFluor 710 50 tests -
15413392 200 Test PCP-eFluor 710 Each -
15581947 100 Tests FITC, Propidium Iodide 100 tests 488, 535/520, 617
15501957 20 Tests FITC, Propidium Iodide 20 tests 488, 535/520, 617
15521957 300 Tests FITC, Propidium Iodide 300 tests 488, 535/520, 617
15521207 200 Tests PE, 7-AAD 200 tests -
15531207 50 Tests PE/Cy7 50 tests -
15541207 200 Tests PE/Cy7 200 tests -
15551947 100 Tests Biotin, Propidium Iodide 100 tests NA, 535/NA, 617
15561947 20 Tests Biotin, Propidium Iodide 20 tests NA, 535/NA, 617
15571947 300 Tests Biotin, Propidium Iodide 300 tests NA, 535/NA, 617
12760170 50 Tests FITC, Propidium Iodide Each 488, 535/520, 617
15581187 50 Tests APC, Propidium Iodide 50 tests 650, 535/660, 617
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 18 options available.
18 options
This item is not returnable. View return policy
Product Code. 14819780 Supplier Invitrogen™ Supplier No. 88810272

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This item is not returnable. View return policy

Description

Includes

  • 10X Binding Buffer
  • Annexin V PE : 5μL/test, 50 tests Protect from light.
  • 7-AAD Viability Staining Solution : 5μL/test, 100 tests Protect from light.
  • Cat. No. 88-8102-74:
  • 10X Binding Buffer
  • Annexin V PE : 5μL/test, 200 tests Protect from light.
  • 7-AAD Viability Staining Solution : 5μL/test, 2x100 tests Protect from light.

Kits utilize viability dye-conjugated annexin V to label phosphatidylserine (PS), an early marker of apoptosis, on the extracellular membrane.

Easily distinguish cells undergoing early and late apoptosis with eBioscience and Annexin V Apoptosis Detection kits for flow cytometry analysis. These kits utilize viability dye-conjugated annexin V to label phosphatidylserine (PS), an early marker of apoptosis, on the extracellular membrane. In late-stage apoptosis, as cell integrity is lost, both annexin V and the viability dye traverse and bind PS on the interior of the plasma membrane.

Annexins are a family of calcium-dependent phospholipid-binding proteins that preferentially bind phosphatidylserine (PS). Under normal physiologic conditions, PS is predominantly located in the inner leaflet of the plasma membrane. Upon initiation of apoptosis, PS loses its asymmetric distribution across the phospholipid bilayer and is translocated to the extracellular membrane leaflet marking cells as targets of phagocytosis. Once on the outer surface of the membrane, PS can be detected by fluorescently labeled annexin V in a calcium-dependent manner.

In early-stage apoptosis, the plasma membrane excludes viability dyes such as propidium iodide (PI), 7-AAD, or fixable viability dyes such as eFluor 450. These cells will stain with annexin V but not a viability dye, thus distinguishing cells in early apoptosis. However, in late-stage apoptosis, the cell membrane loses integrity, thereby allowing annexin V to also access PS in the interior of the cell. A viability dye can be used to resolve these late-stage apoptotic and necrotic cells (annexin V, viability dye-positive) from the early-stage apoptotic cells (annexin V positive, viability dye-negative).

Current viability stains available with the eBioscience Annexin V Apoptosis Detection kits include FITC, eFluor 450, allophycocyanin (APC), phycoerythrin (PE), PE-cyanine7, and biotin.

TRUSTED_SUSTAINABILITY

Specifications

Description eBioscience Annexin V Apoptosis Detection Kit PE
Quantity 50 Tests
Kit Contents Contains:
• Annexin V Binding Buffer (10X), 1 bottle (30 mL)
• Annexin V PE, 1 vial (0.25 mL)
• 7-AAD Viability Staining Solution, 1 vial (0.5 mL)

Note: Fixable Viability Dye eFluor 450 is not recommended for use with Annexin V Apoptosis Detection Kits.
Product Type Apoptosis Detection Kit
Content And Storage Handling Conditions:
Store at 2-8°C. Avoid temperature fluctuations.
Shipping Condition Wet Ice
Conjugate PE, 7-AAD
For Use With (Equipment) Fluorescence Microscope, Flow Cytometer
Product Title
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