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  5. Invitrogen™ Dehydroepiandrosterone Sulfate (DHEA-S) Competitive ELISA Kit

Invitrogen™ Dehydroepiandrosterone Sulfate (DHEA-S) Competitive ELISA Kit

Product Code. p-200006745
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Quantity:
10 x 96 Tests
96 Tests
Unit Size:
Pack of 10
Pack of 96
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Product Code. 15880322

Brand: Invitrogen™ EIADHEA

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Description

ELISA

The Dehydroepiandrosterone Sulfate (DHEA-S) ELISA quantitates DHEA-Se in serum,plasma,urine,saliva,dried fecal extracts or cell culture medium.

Principle of the method

The Dehydroepiandrosterone sulfate (DHEA-S) Competitive ELISA research-use-only kit uses a specifically generated antibody to measure Dehydroepiandrosterone sulfate (DHEA-S) in serum,plasma,urine,and saliva samples,and in fecal extracts. The kit will also quantitatively measure DHEA-S present in tissue culture media samples. A DHEA-S standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture sheep antibodies. A DHEA-S-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to DHEA-S to each well. After a 2-hour incubation the plate is washed and substrate is added. The substrate reacts with the bound DHEA-S-peroxidase conjugate. After a short incubation,the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity,specificity,precision,and lot-to-lot consistency. See manual for more information on validation.

Dehydroepiandrosterone sulfate (DHEA-S) is the major C19 steroid secreted by the adrenal cortex and a precursor in testosterone and estrogen biosynthesis. DHEA-S is identical across all species and so this kit should measure DHEA-S from all sources. It is produced by the addition of a sulfate group to dehydroepiandrosterone (DHEA), catalyzed by the sulfotransferase enzymes, SULT1A1 and SULT1E1, which also produce estrone sulfate from estrone. DHEA sulfate can also be back-converted to DHEA through the action of steroid sulfatase.
TRUSTED_SUSTAINABILITY

Specifications

Assay Range 96 to 60,000 pg/mL
Assay Sensitivity 90.9 pg/mL
Description Dehydroepiandrosterone sulfate (DHEA-S) Competitive ELISA Kit
Assay Multiplex assay on the Luminex Platform
Conjugate HRP
Detection Method Absorbance at 450 nm
Product Type ELISA
Protein Family Steroid hormone
Sample Type Fecal Extract, Plasma, Saliva, Serum, Supernatant, Urine
Sufficient For 96 assays
Target Species Human
For Use With (Application) ELISA, Protein Assays, Aktivity Assays, Protein Biology, Reproductive Biology, Inflammation, Oxidative Stress, Metabolism, Cell Signaling, Epigenetics, Hematology, DNA Damage, Cancer Biology, Immunology, Neuroscience, Neurobiology, Steroid Hormone Detecti
For Use With (Equipment) Colorimetric Microplate Reader
Interassay CV 8.4%
Intraassay CV 7
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, HRP-Conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 96 Tests
Regulatory Status RUO
Research Discipline Reproductive Biology
Sample Volume Fecal Extract 50 μL, Plasma 1 μL, Saliva 25 μL, Serum 1 μL, Supernatant 50 μL, Urine 25 μL
Storage Requirements -20°C
Target DHEA-S
Test Time 1 hrs 20 mins
Total Assay Time 2 hrs 30 mins
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