Compbead Plus anti-Mouse Ig, κ/Negative Control (BSA) Compensation Plus (7.5μm) Particles Set, BD

The BD CompBead Plus Anti-Mouse Ig, kappa beads are polystyrene particles used to establish fluorescence compensation settings for multicolor flow cytometric analyses. The kit provides two types of particles, the BD CompBead Plus Anti-Mouse Ig, kappa particles, which bind any mouse kappa light chain-bearing immunoglobulin, and the BD CompBead Plus Negative Control (BSA) particles, which have no binding capacity. When mixed together with a fluorochrome-conjugated mouse antibody, the BD CompBead Plus provides distinct positive and negative (background fluorescence) stained populations which can be used to set compensation levels manually or using instrument set-up software. Since the compensation adjustments are made using the same fluorochrome-labeled antibody to be used in the experiment, this method allows the investigator to more accurately establish compensation corrections for spectral overlap for any combination of fluorochrome-labeled antibodies (without having to use valuable tissue samples or hard-dyed beads with potentially mismatched fluorescence spectra). Use of the BD™ CompBead Plus is highly recommended for use in all experiments using tandem dye (i.e., PE-Cy7, APC-Cy7, etc.) conjugates, which may have distinct spectral characteristics for each conjugate.

Flow Cytometry