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  5. Invitrogen™ Monkey CD31/PECAM (Soluble) ELISA Kit

Invitrogen™ Monkey CD31/PECAM (Soluble) ELISA Kit

Product Code. p-7092143
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Quantity:
96 Tests
Unit Size:
96 tests
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Product Code. 15582097

Brand: Invitrogen™ BMS651

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Description

ELISA

The Monkey Soluble CD31/Platelet Endothelial Cell Adhesion Molecule (MK sCD31/PECAM) ELISA quantitates Mk sCD31/PECAM in monkey(baboon) serum or cell culture medium. The assay will exclusively recognize both natural and recombinant Mk sCD31/PECAM. Principle of the method The Mk sCD31/PECAM solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system.
TRUSTED_SUSTAINABILITY

Specifications

Assay Range 0.48 to 30.0 U/mL
Assay Sensitivity 0.06 U/mL
Conjugate HRP
Product Type ELISA
Sample Type Serum, Supernatant
Target Species Monkey
For Use With (Application) ELISA
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 101003471
Intraassay CV 2
Kit Contents Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, HRP-conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Controls, Adhesive Plate Covers
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Serum 10 μL, Supernatant 10 μL
Synonym Leukocyte surface protein, CD31, EndoCAM, HEC7
Target CD31 (PECAM-1)
Test Time 1 hrs 20 mins
Total Assay Time 3 hrs 10 mins
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