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CD28 Monoclonal Antibody (CD28.6), Functional Grade, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

127.00 EUR - 358.00 EUR

Specifications

Antigen CD28
Clone CD28.6
Concentration 1 mg/mL
Applications Flow Cytometry, Functional Assay, Neutralization, Western Blot
Classification Monoclonal
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Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15258417
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Affymetrix eBioscience
16-0288-81
50 μg
127.00 EUR
50µg
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Estimated Shipment: 10-04-2024
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15268417
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Affymetrix eBioscience
16-0288-85
500 μg
358.00 EUR
500µg
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Description

Description

Description: The CD28.6 monoclonal antibody reacts with the human CD28 molecule, a 44 kDa homodimer expressed by thymocytes, mature T cells and plasma cells. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) and is a potent costimulator of T cells. Signaling through CD28 augments IL-2 and IL-2 receptor expression as well as cytotoxicity of CD3-activated T cells. The CD28.6 antibody has been reported for use in immunoblotting (WB) and neutralization studies and costimulation in the production of IL-2. Applications Reported: The CD28.6 antibody has been reported for use in flow cytometric analysis, and immunoblotting (WB). The recommended titer for WB is (∽1 μ/mL). CD28.6 has also been reported in in vitro functional assays, both blocking and co-stimulatory activities have been reported for this clone (please see references below). Applications Tested: TheCD28.6 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.

CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. CD28 is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes. Moreover, CD28 is the critical T cell costimulatory receptor that provides the cell the important second activation signal by binding CD80 and CD86 which are expressed by antigen presenting cells. In addition to its co-stimulation role, CD28 functions by preventing T cells from entering an anergic-hyporesponsive state or from undergoing premature apoptotic cell death. In murine peripheral lymphoid organs and in the blood, all CD4+ and CD8+ T cells express CD28. In the thymus, CD28 expression is highest on immature CD3-, CD8+ and CD4+8+ cells, and on CD4-8- cells that express alpha/beta and gamma/delta TCR. The level of CD28 on mature CD4+ and CD8+ alpha/beta TCR+ thymocytes is two- to fourfold lower than on the immature cells. Diseases associated with CD28 dysfunction include mycosis fungiodes and Sezary's Disease.
Specifications

Specifications

CD28
1 mg/mL
Monoclonal
Liquid
RUO
PBS with no preservative; pH 7.2
antigen CD28; CD28; CD28 antigen; CD28 antigen (Tp44); CD28 isoform; CD28 isoform 2; Cd28 molecule; CD28 precursor protein; CD28 protein; CD28 protein precursor; CD28RNA; cell surface protein; CHT28; contains partial extracellular domain; costimulatory molecule B7 receptor CD28; EGK_04709; MGC138290; sCD28; soluble CD28; T44; T-cell costimulatory molecule CD28; T-cell costimulatory molecule Tp44; T-cell-specific surface glycoprotein CD28; T-cell-specific surface glycoprotein CD28 homolog; TP44
Cd28
Primary
4° C
Cd28
CD28.6
Flow Cytometry, Functional Assay, Neutralization, Western Blot
Functional Grade
Mouse
Human
P10747
940
IgG2a κ
Affinity chromatography
Antibody
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