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AcroBiosystems Biotinylated Human CD209 / DC-SIGN Protein, AvitagTM,His Tag

Product Code. 31452653
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200 μg
25 μg
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31452653 25 μg
31453011 200 μg
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Product Code. 31452653 Supplier AcroBiosystems Supplier No. CD9H82Q325ug

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Description

Recombinant Protein

CD209 is also known as CLEC4L, DC-SIGN and CD209 antigen. Pathogen-recognition receptor expressed on the surface of immature dendritic cells (DCs) and involved in initiation of primary immune response. On DCs it is a high affinity receptor for ICAM2 and ICAM3 by binding to mannose-like carbohydrates. May act as a DC rolling receptor that mediates transendothelial migration of DC presursors from blood to tissues by binding endothelial ICAM2. Seems to regulate DC-induced T-cell proliferation by binding to ICAM3 on T-cells in the immunological synapse formed between DC and T-cells.

Specifications

Accession Number Uniprot
Characterization CD209 Structure This protein carries an Avi tag (AvitagTM) at the N-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 43.0 kDa. The protein migrates as 47-50 kDa when calibrated against under reducing (R) condition (SDS-PAGE) due to glycosylation.
Format Powder
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4 with trehalose as protectant. Contact us for customized product form or formulation.
Quality Control Testing Less than 1.0 EU per μg by the LAL method / rFC method.
Quantity 25 μg
Source Biotinylated Human CD209 Protein, Avitag,His Tag (CD9-H82Q3) is expressed from human 293 cells (HEK293). It contains AA Gln 59 - Ala 404 (Accession # ). Predicted N-terminus: Gly Request for sequence
Biological Activity Immobilized SARS-CoV-2 Spike S1, His Tag (Cat. No. S1N-C52Ht) at 1 μg/mL (100 μL/well) can bind Biotinylated Human CD209 Protein, Avitag,His Tag (Cat. No. CD9-H82Q3) with a linear range of 0.07-0.313 μg/mL (QC tested).
Species Human
Content And Storage -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin Level Less than 1.0 EU per μg by the LAL method / rFC method.
Purity or Quality Grade 0.95
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