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SYBR™ Power SYBR™ Green PCR Master Mix
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Quantity:
1 x 1 mL
1 x 5 mL
2 x 5 mL
5 x 5 mL
10 x 5 mL
1 x 50 mL
Tamaño de la unidad:
1000 reactions
200 reactions
2000 reactions
40 reactions
400 reactions
Each
Descripción
Includes
SYBR™ Green 1 Dye, AmpliTaq Gold DNA Polymerase LD, dNTPs with dUTP/dTTP blend, Passive Reference 1, and optimized buffer components
Requires
Template and primers
Get superior nucleic acid quantitation with Applied Biosystems™ Power SYBR™ Green Master Mix.
- Everything needed for SYBR™ Green dye-based PCR amplification and detection in a convenient single-tube format
- Power SYBR™ Green PCR Master Mix contains all of the components (excluding the template and primers) for superior SYBR™ Green reagent-based real-time PCR
- The optimized formulation contains highly purified AmpliTaq Gold™ DNA Polymerase, LD to offer increased sensitivity
- Contains a blend of dTTP/dUTP that is compatible with AmpErase™ UNG to minimize carryover contamination
- Includes a proprietary version of ROX™ dye, an internal passive reference, to normalize non-PCR–related fluorescence fluctuations
- This minimizes well-to-well variability that can result from a variety of causes, such as pipetting error and sample evaporation
- Power SYBR™ Green reagent-based PCR chemistry easily replaces the existing SYBR™ Green PCR Master Mix in Applied Biosystems™ protocols using the same setup and thermal cycling conditions
- Store at -20°C
Información de órdenes
Shipping Condition: Wet Ice
Especificaciones
Especificaciones
| Concentration | 2X |
| Content And Storage | The 2X mix contains SYBR™ Green 1 Dye, AmpliTaq Gold™ DNA Polymerase LD, dNTPs with dUTP/dTTP blend, Passive Reference 1, and optimized buffer components. Contains 1 × 50 mL bottle, sufficient for 1000 reactions of 50 μL each. Store at -20°C. Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA). |
| Detection Method | SYBR |
| Format | Bottle |
| GC-Rich PCR Performance | High |
| PCR Method | qPCR |
| Polymerase | AmpliTaq Gold DNA Polymerase, AmpliTaq Gold LD |
| Reaction Speed | Standard |
| For Use With (Equipment) | 7300 System, 7500 System, 7700 System, 7900HT Fast System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System |
| Label or Dye | SYBR Green |
| Mostrar más |
For Research Use Only. Not for use in diagnostic procedures.
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