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Applied Biosystems™ GeneAmp™ RNA PCR Core Kit
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Includes
MuLV Reverse Transcriptase, AmpliTaq DNA Polymerase, RNase Inhibitor, dNTPs, GeneAmp 10X PCR Buffer, MgCl2 Solution, Oligo d(T)16, and Random Hexamers
The Applied Biosystems GeneAmp RNA PCR Core Kit contains all components necessary to reverse transcribe RNA to cDNA using MuLV(recombinant Moloney murineleukemia virus) reverse transcriptase and to subsequently amplify synthesized cDNA using PCR process.
- RNA reverse transcription and cDNA amplification combined in a single tube provides exceptional sensitivity
- Kit protocol enables users to perform a variety of studies including: amplification of cDNA derived from all types of RNA sequences,analysis of gene expression at the RNA level, and detection of specific RNA species present at very low copy number
- Easy to Use: Both the reverse transcription and amplification reactions take place in a single tube with buffers and reagents co-optimized for these sequential reactions
- Final product size: 5kb or less
- Sufficient for 100 reverse transcription reactions, 20μL each and 100 PCR amplifications, 100μL each
See user's manual or package insert for limited label license and trademark information.
PCR & Real-Time PCR, RT-PCR, Reverse Transcription, Two-Step RT-PCR
Order Info
Shipping Condition: Dry ice
Specifications
Specifications
| Content And Storage | MuLV Reverse Transcriptase, AmpliTaq DNA Polymerase, RNase Inhibitor, dNTPs, GeneAmp 10X PCR Buffer, MgCl2 Solution, Oligo d(T)16, and Random Hexamers. Store at -20deg.C. |
| Detection Method | Primer-probe |
| Quantity | 100 reactions each (RT and PCR) |
| For Use With (Application) | RT-PCR |
| Sample Type | RNA |
| Volume | 20μL (RT), 100μL (PCR) |
For Research Use Only. Not for human or animal therapeutic or diagnostic use.
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