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Molecular Probes™ APO-BrdU™ TUNEL Assay Kit, with Alexa Fluor™ 488 Anti-BrdU

Product Code. 10043102
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Quantity:
60 Assays
Förpackningsstorlek:
5mL
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Produktkod. 10043102

Brand: Molecular Probes™ A23210

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Beskrivning

APO-BrdU™ TUNEL Assay Kit, with Alexa Fluor™ 488 Anti-BrdU

In collaboration with Phoenix Flow Systems, we offer the APO™-BrdU TUNEL Assay Kit which provides all the materials necessary to label and detect the DNA strand breaks of apoptotic cells. When DNA strands are cleaved or nicked by nucleases, a large number of 3´hydroxyl ends are exposed. In the APO-BrdU assay, these ends are labeled with BrdUTP and terminal deoxynucleotidyl transferase (TdT) using the TUNEL technique. Once incorporated ino the DNA, BrdU is detected using a bright and photostable green-fluorescent Alexa Fluor™ 488 dye-labeled anti-BrdU antibody.
TRUSTED_SUSTAINABILITY

Specifikationer

Color Green
Content And Storage Contains positive control cells (5 mL) and negative control cells (5 mL) of a fixed human lymphoma cell line, 1 vial of TdT (45 μL), 1 vial of BrdUTP (480 μL), 1 vial of anti-BrdU Ms mAb Alexa Fluor™ 488 conjugate (350 μL), 1 bottle of PI/Rnase A staining buffer (30 mL), 1 vial of reaction buffer (0.6 mL), 1 bottle of wash buffer (120 mL) and 1 bottle of rinse buffer (120 mL).

Store as directed.
Detection Method Fluorescence
Format Tube
Excitation/Emission 490/525
Flow Cytometer Laser Lines 488
For Use With (Application) Flow Cytometry
For Use With (Equipment) Fluorescence Microscope, Flow Cytometer
Label or Dye Alexa Fluor™ 488
Product Type TUNEL Assay Kit
Product Line APO-BrdU
Quantity 60 Assays
Species Mouse
Target BrdU
Label Type Alexa Fluor Dyes
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Produktbeteckning
  • APO-BrdU TUNEL Assay Kit, with Alexa Fluor 488 Anti-BrdU
 Signalord
  • Danger
 Riskkategorier
  • Serious eye damage/eye irritation Category 2
  • Flammable liquid Category 2
 Faroangivelser
  • H225-Highly flammable liquid and vapour.
  • H319-Causes serious eye irritation.
 Skyddsangivelse
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P233-Keep container tightly closed.
  • P240-Ground/bond container and receiving equipment.
  • P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
  • P242-Use only non-sparking tools.
  • P243-Take precautionary measures against static discharge.
  • P264-Wash hands thoroughly after handling.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P337+P313-If eye irritation persists: Get medical advice/attention.
  • P370+P378-In case of fire: Use dry sand, dry chemical or alcohol-resistant foam to extinguish.
  • P403+P235-Store in a well-ventilated place. Keep cool.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Kompletterande information
  • MIXTURE LIST-Contains : ethanol

For Research Use Only. Not for use in diagnostic procedures.

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