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Invitrogen™ PureLink™ miRNA Isolation Kit
The Invitrogen PureLink miRNA Isolation Kit provides a simple, reliable, and rapid column-based method for isolating high-quality total microRNA (miRNA) from a wide variety of sources without the need for hazardous reagents such as phenol.
Brand: Invitrogen™ K157001
Additional Details : Weight : 0.27000kg
Description
The Invitrogen PureLink miRNA Isolation Kit provides a simple, reliable, and rapid column-based method for isolating high-quality total microRNA (miRNA) from a wide variety of sources without the need for hazardous reagents such as phenol. The kit provides a rapid and convenient method for purifying small RNA molecules from animal and plant cells, as well as from bacteria and yeast samples.
- Purify small RNA molecules with a novel enrich/bind/elute procedure
- Prepare enriched microRNA samples typically in less than 15 minutes
- Minimize contaminating large RNA molecules and genomic DNA
Rapid and Convenient Column Purification Procedure for small RNAs
The Invitrogen PureLink miRNA Isolation Kit is specifically designed to purify small RNA molecules, including miRNAs and siRNAs, from a variety of samples typically in less than 15 minutes. The kit utilizes a silica-based two-column system to enrich exclusively for small RNAs. RNAs greater than approximately 200 nucleotides are bound to the first column. Ethanol is then added to the flow-through in order to increase the binding affinity of small RNAs to the glass fiber membrane in the second column. Once bound to the second column, small RNA molecules are then eluted, ready-for-use, and free of large RNA molecules such as 28S rRNA, 18S rRNA, and mRNA, allowing for focused expression analysis studies of small RNA molecules
Purified RNA is Suitable for Use with a Variety of Downstream Applications and Products:
The miRNA purified using the Invitrogen PureLink miRNA Isolation Kit is suitable for downstream applications including RNA sequencing, microarray analysis, real-time PCR (qPCR), reverse transcription real-time PCR (RT-qPCR), and northern blotting
Not high-throughput compatible (Manual)
Applications
DNA and RNA Purification and Analysis, RNA Extraction, RNAi, RNAi, Epigenetics and Non-Coding RNA Research, miRNA and Non-Coding RNA Analysis, miRNA Isolation, microRNA Isolation, siRNA
Order Info
Shipping Condition: Room temperature
Specifications
| • 45 mL Binding Buffer L3; store at room temperature • 10 mL Wsh Buffer W5; room temperature • 3 mL Buffer W4; room temperature • 5 mL RNase-free Water; room temperature • 50 Spin Cartridges with Collection Tubes; room temperature • 50 Wash Tubes (2 mL); room temperature • 50 Recovery Tubes (1.7 mL); room temperature |
|
| 20 min. (After sample homogenization) | |
| Bacteria, Cells, Plant, Tissue, Yeast, Dicing Reaction | |
| Not High-throughput Compatible (Manual) | |
| 25 Preps | |
| Room Temperature | |
| Up to 6 μg |
| Silica Spin Column | |
| 50–100 μL | |
| Small RNA | |
| qRT-PCR, Microarray Analysis, Northern Blotting | |
| Mini | |
| Bacteria: ≤106 cells Cells: 106 Plant: varies Tissue: ≤100 mg Yeast: 1 mL Dicing Reaction: 300 μL |
For Research Use Only. Not for use in diagnostic procedures.