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Invitrogen™ MEGAscript™ T7 Transcription Kit
Description
Includes
T7 Enzyme Mix, 10X Reaction Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate Stop Solution, Lithium Chloride Precipitation Solution, and Gel Loading Buffer II, Nuclease-free Water
The MEGAscript T7 Kit is an ultra-high yield in vitro transcription kit. The high yields are achieved by modifying typical transcription reaction conditions so that very high nucleotide concentrations can be effectively used. The T7 Enzyme Mix and the 10X Reaction Buffer are specifically calibrated for each lot and RNA polymerase.
Using the MEGAscript T7 Kit
The MEGAscript T7 Kit contains in vitro transcription reaction components and a control template. The kit will yield a total of 3–5 mg of RNA (approximately 100 μg of RNA or more per reaction) from the control template supplied with the kit. This corresponds to 400–650 moles of RNA for each mole of template. Smaller templates typically yield a lower mass and a higher molar yield of product. Novel transcription reaction conditions and a patented, high-yield technology allow the synthesis of 10–50 times the amount of RNA produced by conventional transcription reactions.
Applications
The MEGAscript T7 Kit is intended for the synthesis of large amounts of unlabeled or low specific activity RNA for a variety of uses, including in vitro translation, antisense/microinjection studies, and isolation of RNA binding proteins. In large-scale transcription reactions, the concentration of all four nucleotides is high, well above the Km for the enzyme. The MEGAscript T7 Kit typically yields over 10 times more RNA than conventional in vitro transcription reactions. The kit is not recommended for synthesis of high specific activity probes.
Accessory products
MessageAmp aRNA amplification kits are powered by MEGAscript technology and have been developed especially for the amplification of cRNA prior to array analysis. To purify in vitro transcription products from free nucleotides, buffer components, and enzymes, use the MEGAclear™ Kit (Cat. No. AM1908) and MEGAclear-96 Kit (Cat. No. AM1909).
RNA yield: 100 μg
Specifications
Specifications
| Content And Storage | T7 Enzyme Mix, 10X Reaction Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate Stop Solution, Lithium Chloride Precipitation Solution, and Gel Loading Buffer II are all stored at –20°C. Nuclease-free Water may be stored at any temperature. |
| Final Product Type | Synthetic mRNA, mRNA |
| Format | Kit |
| Sample Type | mRNA, Synthetic mRNA |
| For Use With (Application) | In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products |
| No. of Reactions | 25 Reactions |
| Product Line | Ambion, MEGAscript |
| Product Type | T7 In Vitro Transcription kit |
| Quantity | 25 Reactions |
| Promoter | T7 |
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Safety and Handling
- MEGASCRIPT T7 KIT 25 RXNS EACH
- Danger
- Acute toxicity Category 4
- Carcinogenicity Category 2
- Serious eye damage/eye irritation Category 2
- Reproductive toxicity Category 1B
- Skin corrosion/irritation Category 2
- Specific target organ toxicity after repeated exposure Category 2
- H302-Harmful if swallowed.
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
- H351-Suspected of causing cancer.
- H360-May damage fertility or the unborn child.
- H373-May cause damage to organs.
- P201-Obtain special instructions before use.
- P202-Do not handle until all safety precautions have been read and understood.
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P264-Wash hands thoroughly after handling.
- P270-Do not eat, drink or smoke when using this product.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P308+P313-IF exposed or concerned: Get medical advice/attention.
- P314-Get medical advice/attention if you feel unwell.
- P332+P313-If skin irritation occurs: Get medical advice/attention.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
- MIXTURE LIST-Contains : formamide, lithium chloride
For Research Use Only. Not for use in diagnostic procedures.
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