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Thermo Scientific™ ABsolute QPCR Mix, SYBR Green, fluorescein

Product Code. 11842063
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:
400 Reactions
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Product Code. 11842063

Brand: Thermo Scientific™ AB1220A

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Description

Optimized for SYBR Green chemistry and contains all the components necessary to perform quantitative PCR, with the exception of template and primers.

The 2X qPCR SYBR Green Mix contains optimal levels of active SYBR Green I dye and Thermo-Start DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets.

ABsolute qPCR SYBR Green Mixes are available with the option of included high ROX reference dye concentration, Low ROX concentration, Fluorescein or no reference dye. A master mix optimized for capillary instruments is also available.

  • Thermo Scientific ABsolute qPCR SYBR Green Mixes are optimized for SYBR Green chemistry and contain all the components necessary to perform quantitative PCR, with the exception of template and primers
  • The 2X qPCR SYBR Green Mix contains optimal levels of active SYBR Green I dye and Thermo-Start DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets
  • ABsolute qPCR SYBR Green Mixes are available with the option of included high ROX reference dye concentration, low ROX concentration, fluorescein or no reference dye
  • A master mix optimized for capillary instruments is also available

Highlights

  • High Sensitivity & Specificity – The master mixes contain our patented hot start enzyme Thermo-Start DNA Polymerase
  • Optimised buffer containing SYBR Green I
  • Reproducibility – All master mixes undergo extensive QC to ensure batch to batch consistency
  • Store at -20°C
TRUSTED_SUSTAINABILITY

Specifications

Concentration 2X
Content And Storage Contains:
• 1 x 5 mL ABsolute QPCR SYBR Green Fluorescein Mix (2X)
•100 μL MgCl2 (1M)
Sufficient for 400 x 25 μL reactions.

Store at -20°C
Detection Method SYBR
GC-Rich PCR Performance High
PCR Method qPCR
Polymerase Taq DNA Polymerase
Reaction Speed Standard
For Use With (Equipment) BioRad iCycler iQ, BioRad MyiQ, BioRad iQ5
Product Line ABsolute
Product Type Real Time PCR SYBR Master Mix
Sufficient For 400 Reactions of 25 μL Each
For Use With (Application) Gene Expression
Compatibility qPCR Cyclers
No. of Reactions 400 Reactions
Sample Type DNA, dsDNA
Volume 5 mL
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For Research Use Only. Not for use in diagnostic procedures.

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