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R&D Systems™ Recombinant Human Sonic Hedgehog/Shh (C24II) Animal-Free

Product Code. 18709636
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Quantity:
100 μg
25 μg
500 μg
Unit Size:
100µg
25µg
500µg
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Product Code. 18709636

Brand: R&D Systems™ AFL1845100

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This item is not returnable. View return policy

Description

Also available, GMP Shh Protein, Catalog # 1845-GMP! Animal-free and manufactured under GMP guidelines for therapeutic manufacturing applications.

Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. Nakamura, T. et al. (1997) Biochem. Biophys. Res. Commun. 237:465. The ED50 for this effect is 0.1-0.4 μg/mL.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number NP_000184.1
For Use With (Application) Bioactivity
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
Gene ID (Entrez) 6469
Molecular Weight (g/mol) M.W.-observed: 22 kDa, reducing conditions, M.W.-theroretical: 20 kDa
Quantity 100 μg
Source E. coli-derived human Sonic Hedgehog/Shh protein Cys24-Gly197 (Cys24Ile-Ile), with an N-terminal Met Produced using non-animal reagents in an animal-free laboratory.
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
Endotoxin Concentration <0.01 EU / 1 μg of the protein by the LAL method.
Gene Alias HHG1, HHG-1, HLP3, HPE3, MCOPCB5, MCOPCB5sonic hedgehog (Drosophila) homolog, Shh, ShhNC, SMMCI, SMMCIsonic hedgehog homolog (Drosophila), sonic hedgehog, sonic hedgehog homolog, sonic hedgehog protein, TPT, TPTPS
Conjugate Unconjugated
Reconstitution Reconstitute at 100-200 μg/mL in PBS, and allow up to 24 hours (at 2°C to 8°C for complete reconstitution.)
Purity or Quality Grade >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Protein Sonic Hedgehog/Shh
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