TdB Labs AB 6 Mda Fluorescein Hyaluronic Acid

Product Overview: Fluorescein-hyaluronic acid 6 MDa is a high molecular weight hyaluronic acid conjugated with fluorescein, providing strong green fluorescence for biological imaging, molecular tracking, and barrier function studies. With an approximate molecular weight of 6 million Daltons, this product is designed for modeling the behavior of extremely large biomolecules and polymers in biological environments.

• Features:
  • High Molecular Weight: Approximately 6 MDa, offering properties similar to naturally occurring extracellular matrix components.
  • Green Fluorescence: Excitation maximum at 495 nm and emission maximum at 519 nm, compatible with standard FITC filter settings.
  • Water-Soluble: Readily dissolves in aqueous solutions, facilitating easy preparation for biological studies.
  • Biocompatible Backbone: Hyaluronic acid is naturally occurring and well-tolerated in biological systems, supporting use in live cell and tissue models.
  • Controlled Degree of Labeling: Ensures bright fluorescence while preserving native polymer characteristics and behavior.
• Benefits:
  • Ideal for ECM and Barrier Studies: Models large molecule transport in extracellular matrix environments and across biological barriers.
  • Visualizes Molecular Movement: Tracks the diffusion and distribution of high molecular weight biomolecules in tissue and hydrogel systems.
  • Suitable for Live and Fixed Imaging: Can be used in both dynamic live-cell setups and after fixation for endpoint assays.
  • Represents Native Biological Behavior: High molecular weight closely mimics natural hyaluronic acid found in tissues, enabling physiologically relevant studies.
  • Stable, Bright Fluorescence: Provides consistent, high-intensity signal for reliable imaging and quantitative analysis.
• Applications:
  • Extracellular Matrix Research: Used to model diffusion and retention of large biomolecules in native and engineered tissue systems.
  • Barrier Permeability Studies: Tracks the ability of macromolecules to cross biological barriers like endothelial and epithelial layers.
  • Tissue Engineering and Regenerative Medicine: Studies integration and movement of large molecules in scaffold-based and hydrogel-based systems.
  • Drug Delivery Research: Models nanoparticle or macromolecular therapeutic diffusion through tissues.
  • In Vivo and In Vitro Imaging: Used to visualize macromolecule distribution in animal models and cultured tissues.
• Technical Specifications:
  • Fluorescence: Excitation maximum at 495 nm, emission maximum at 519 nm.
  • Molecular Weight: Approximately 6 million Daltons (6 MDa).
  • Appearance: Light-sensitive, greenish dry powder, fully water-soluble.
  • Labeling: Fluorescein conjugation optimized for strong fluorescence with minimal alteration of HA's biological properties.
• Storage and Stability:
  • Store dry and protected from light at 2°C to 8°C for optimal stability.
  • Stable for extended periods when stored under recommended conditions.
• Packaging and Availability:
  • Supplied as dry powder for ease of storage and handling.
  • Available in various research packaging sizes to support different experimental needs.