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R&D Systems™ Recombinant Human Wnt-2/sFRP-1 Complex Protein

Product Code. 18715856
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Quantity:
10 μg
Unit Size:
10µg
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Product Code. 18715856

Brand: R&D Systems™ 11117WN010

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This item is not returnable. View return policy

Description

Measured by its ability to activate Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 10.0-150 ng/mL.
TRUSTED_SUSTAINABILITY

Specifications

For Use With (Application) Bioactivity
Formulation Lyophilized from a 0.2 μm filtered solution in MOPS and NaCl with Trehalose.
Gene ID (Entrez) 7472
Molecular Weight (g/mol) M.W.-observed: 30-45 kDa, under reducing conditions., M.W.-theroretical: 38 kDa (Wnt-2) & 33 kDa (sFRP-1)
Quantity 10 μg
Source Chinese Hamster Ovary cell line, CHO-derived human Wnt-2 protein Human Wnt-2 (Ser26-Thr360) (C-terminus) Accession # P09544.1 Human sFRP-1 (Ser32-Lys314) (C-terminus) Accession # AAB70793.1 (N-terminus)
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
Endotoxin Concentration <0.10 EU / 1 μg of the protein by the LAL method.
Gene Alias FrzA, INT1L1, INT1L1secreted growth factor, Int-1-like protein 1, Int-1-related protein, IRP, IRPprotein Wnt-2, SARP-2, sFRP1, wingless-type MMTV integration site family member 2, Wnt2
Conjugate Unconjugated
Reconstitution Reconstitute at 100 μg/mL in PBS.
Purity or Quality Grade >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Protein Wnt-2
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