Biochemicals and Reagents

Thermo Scientific™ pBR322 DNA/BsuRI (HaeIII) Marker

Perform sizing and approximate quantification of small double-stranded DNA fragments in agarose and non-denaturing polyacrylamide gels. 50 UG DNA LADDERS, PBR322 DNA/BSURI (HAEIII)marker, 5, 0.5ug/µL (supplied with 1mL of 6X DNA

Thermo Scientific™ phiX174 DNA/BsuRI (HaeIII) Marker 9

Size and approximately quantify small double-stranded DNA fragments in agarose and non-denaturing polyacrylamide gels with this conventional marker composed of 11 sharp bands. X5 DNA ladders, phiX174 DNA/BsuRI (HaeIII) marker,

Thermo Scientific™ MassRuler Low Range DNA Ladder, Ready-to-Use, 80-1031 bp

Perform accurate quantification and sizing of DNA fragments by agarose gel electrophoresis with these low range DNA ladders. 1 MG DNA LADDERS, MASSRULER(TM) LOW RANGE DNAladder, ready-to-use, 80-1031 bp, 60.8ng/µL (suppl

Ambion™ SYBR™ Green Cells-to-CT™ Control Kit

Fewer resources used, less hazardous, less waste 100 RXN SYBR GREEN CELLS-TO-CT CONTROL KIT 100REACtions store at -20°C

Invitrogen™ Ambion™ NorthernMax™ 10X Running Buffer

For use in denaturing formaldehyde agarose gel systems 1 LT NORTHERNMAX 10X RUNNING BUFFER 1L STORE AT4°C

Invitrogen™ Ambion™ Nuclease-Free Water (not DEPC-Treated)

Autoclaved, membrane-filtered NUCLEASE-FREE WATER 5 X 100 MLAmbion® Nuclease-free water has not been treated

Applied Biosystems™ AmpliTaq™ Gold 360 DNA Polymerase

Efficiently and reproducibly amplifies long (up to 5 Kb) sequences 5000UNIT AMPLITAQ GOLD 360 DNA POLYMRSE

Thermo Scientific™ GeneJET™ Genomic DNA Purification Kit

Silica membrane-based fast and convenient DNA purification from formalin-fixed paraffin-embedded (FFPE) tissue samples GENEJET FFPE DNA PURIFICATION

Invitrogen™ Ambion™ MEGAclear™-96 Transcription Clean-Up Kit

Quickly cleanup large-scale transcription reactions 96 RXN MEGACLEAR-96 KIT 96REACTIONS STORE AT 4°C

Invitrogen™ ChargeSwitch™ Wash Buffer W12

Use with magnetic beads and column kits 1 LT CHARGESWITCH(TM) WASH BUFFER (W12) 1L STOREat room temperature

Invitrogen™ GeneArt™ Type IIs Assembly Kit

Provides seamless cloning and assembly of up to 8 DNA fragments by simultaneous cleavage and ligation in a single reaction 10RXN GeneArt® Type IIs Assembly Kit, Aar I

Invitrogen™ Ambion™ Conical Tubes (50mL) (bagged)

DNase-free, RNase-free 50 ML CONICAL TUBES (BAGGED)Ambion® RNase-free Conical Tubes are provided

Fisher BioReagents™ Molecular Weight Markers

Suitable for determining the size of DNA fragments X5 Yeast DNA PFGE markers

Thermo Scientific™ Phusion™ Hot Start II High-Fidelity DNA Polymerase

Optimize PCR with one of the most accurate hot start DNA polymerases on the market, featuring extreme specificity and improved performance. X100 UNITS PHUSION HOT START II HIGH-FIDELITY DNA

Invitrogen™ Ambion™ ARCA (Anti-Reverse Cap Analog)

Cap analog for in vitro transcription for capped transcripts generation 40 MMOL ARCA (ANTI REVERSE CAP ANALOG) 40MMOLStore at -80°C

Invitrogen™ Platinum™ Blue PCR SuperMix

Premixed reagents for high-throughput applications PLATINUM BLUE PCR SUPERMIX

Thermo Scientific™ Verso™ cDNA synthesis Kit

Perform robust transcription of RNA to create a complete cDNA pool. VERSO CDNA KIT

Thermo Scientific™ T3 RNA Polymerase

Catalyze the 5'→3' synthesis of RNA on either single-stranded or double-stranded DNA with T73 RNA polymerase. MODIFYING ENZYME, T3 RNA POLYMERASE, 20U/µL (SUPPLied with 2 x 1mL of 5X transcription buffer)

Invitrogen™ Quant-iT™ PicoGreen™ dsDNA Assay Kit

Selectively detect 25pg/mL of dsDNA in presence of ssDNA, RNA, and free nucleotides DSDNA QUANTITATION KIT, PICOGREEN(R), KIT 1 KITStore at 2-8C

Invitrogen™ Ambion™ RNaseZap™ RNase Decontamination Solution

Surface decontaminant that destroys RNases on contact 1500ML RNASEZAP 6 X 250 ML W/MNL

Invitrogen™ E-Gel™ EX Agarose Gels Basic Starter Kit, 2%, EU Version

E-Gel EX Agarose Gels Basic Starter Kit, 2%, EU Version E-GEL EX BASIC STR KIT, 2%, EU

Invitrogen™ Ambion™ PureLink™ RNA Mini Kit

Provides a simple, reliable, and rapid column-based method for isolating high-quality total RNA from a wide variety of sources without the need for hazardous reagents such as phenol X50 PURELINK RNA MINI KIT (12183018A) LITL18 Promotion

Invitrogen™ SYBR™ Safe™ DNA Gel Stain Starter Kit

Developed specifically for reduced mutagenicity – as a safer alternative to ethidium bromide for staining DNA in agarose or acrylamide gels DNA GEL STAIN STARTER KIT 00

Invitrogen™ E-Gel™ Agarose Gels Starter Pak, 1.2% (EU only)

E-Gel™ Agarose Gels Starter Pak, 1.2% (EU only) E-GEL 1.2% STARTER PAK(EU ADAP

Invitrogen™ E-Gel™ 96 High Range DNA Marker

Suitable for sizing doublestranded DNA on all E-Gel™ gel formats 100 RXN E-GEL(R) 96 HIGH RANGE DNA MARKER 100applications Store at -20C

Invitrogen™ Quant-iT™ RNA Assay Kit, Broad Range

Provides an accurate and selective method for quantitation of high-abundance RNA samples Quant-iT RNA Assay Kit, broad range, 1 KitThe new Quant-iT RNA Assay Kit, Broad Range,

Thermo Scientific™ GeneJET™ Plasmid Miniprep Kit

Perform isolation of high quality plasmid DNA from recombinant E. coli cultures with this simple, rapid, and cost-effective system. NUCLEIC ACID PURIFICATION, GENEJET(TM) PLASMIDminiprep kit, includes resusp., lysis, neutraliz.,

Applied Biosystems™ TaqMan™ Fast Advanced Master Mix

TaqMan™ Fast Advanced Master Mix has been designed to match or exceed the performance of standard master mixes, delivering shorter run times (<40 minutes). X5 TAQMAN FAST ADVANCE MMIX (4444964) LITL18 Promotion

Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Gold Buffer and MgCl2

Chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation AMPLITAQ GOLD 5X1000U

Invitrogen™ PureLink™ HiPure Plasmid Filter Midiprep Kit

Purification in 90 minutes PURELINK HIPURE FILTER MIDIpreps