Immunoassay Reagents and Kits
Immunoassay Reagents and Kits
Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.
We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.
ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.
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Invitrogen™ Cell Extraction Buffer
Suitable for use in ELISA and Western blotting
Target Species | Human |
---|---|
Conjugate | Biotin |
Gene Symbol | GMCSF |
Total Assay Time | 24 hr. 30 min. |
Sample Volume | Plasma, 100 μL; Serum, 100 μL; Supernatant, 100 μL |
Assay Sensitivity | 2 pg/mL |
Storage Requirements | 2°C to 8°C |
Assay Range | 2-250 pg/mL |
For Use With (Application) | ELISA |
Test Time | 1 hr. 45 min. |
Accession Number | P10145 |
Regulatory Status | RUO |
Target | Human |
Kit Contents | Capture Antibody: Pre-titrated; purified antibody,Detection Antibody: Pre-titrated; biotin-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,Coating Buffer: 10X PBS ELISA Coating Buffer,Assay Diluent: 5X Concentrated,Detection Enzyme: Pre-titrated Avidin-HRP,Substrate Solution: Tetramethylbenzidine (TMB) Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards.,96-well Plates: Corning Costar flat-bottom plates (included with Cat. Nos. ending in -22 and -86) |
For Use With (Equipment) | Colorimetric Microplate Reader |
Product Type | ELISA Kit |
Gene ID (Entrez) | 3576 |
Sample Type | Plasma,Serum,Supernatant |
Label or Dye | HRP |
Synonym | Interleukin-8, CXCL8, NAP-I |
Invitrogen™ Cytokine 30-Plex Human Panel
Specifically designed for quantifying human cytokines, chemokines and growth factors in serum, plasma, and tissue culture supernatant
Invitrogen™ Automated Maintenance Plate (Luminex™ System)
Compatible with Luminex 100 and 200 Systems
Invitrogen™ TNF alpha Porcine ELISA Kit
Quantifies natural and recombinant swine TNF-α in swine serum, EDTA plasma, buffered solution, or cell culture medium
Target Species | Porcine |
---|---|
Conjugate | Biotin |
Interassay CV | 7.3% |
Intraassay CV | 6% |
Incubation Time | 4 hr. |
Gene Symbol | TNFa |
Total Assay Time | 5 hr. |
Sample Volume | Plasma, 100 μL; Serum, 100 μL; Supernatant, 100 μL |
Assay Sensitivity | <3 pg/mL |
Storage Requirements | 2°C to 8°C |
Assay Range | 23.4-1500 pg/mL |
Marker Gene | TNF-α |
For Use With (Application) | Colorimetric Detection |
Test Time | 1 hr. 20 min. |
Accession Number | P23563 |
Regulatory Status | RUO |
Target | Porcine |
Kit Contents | Pre-coated 96 well plate,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
For Use With (Equipment) | Colorimetric Microplate Reader |
Product Type | ELISA Kit |
Gene ID (Entrez) | 397086 |
Sample Type | Plasma,Serum,Supernatant |
Label or Dye | HRP |
Invitrogen™ Cell Lysis Buffer II
Cell Lysis Buffer II is a high-quality, ready-to-use lysis buffer suitable for the preparation of cell extracts for ELISA, western blotting, and antibody bead immunoassays (Luminex) applications.
Invitrogen™ IL-1 beta Human ELISA Kit
Quantifies natural and recombinant human IL-1β in serum, buffered solution, or cell culture medium
Target Species | Human |
---|---|
Conjugate | Biotin |
Interassay CV | 6.6% |
Intraassay CV | 4.4% |
Gene Symbol | IL-1,IL1-BETA,IL1F2 |
Total Assay Time | 2 hr. 55 min. |
Sample Volume | Serum, 50 μL; Supernatant, 50 μL |
Assay Sensitivity | 1 pg/mL |
Storage Requirements | 2°C to 8°C |
Assay Range | 3.9-250 pg/mL |
For Use With (Application) | Colorimetric |
Test Time | 1 hr. 20 min. |
Accession Number | P01584 |
Regulatory Status | RUO |
Target | Human |
Kit Contents | Pre-coated 96 well plate,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
Precision | 1pg/mL |
For Use With (Equipment) | Colorimetric Microplate Reader |
Product Type | ELISA Kit |
Gene ID (Entrez) | 3553 |
Sample Type | Serum,Supernatant |
Label or Dye | HRP |
Invitrogen™ Interferon alpha (Cell culture) Human ELISA Kit
Measure levels of human interferon alpha (IFNa) in human serum and human plasma samples using 96-well plates.
Measurement | Absorbance at 450nm |
---|---|
Immunoassay Kit Format | Colorimetric ELISA (TMB substrate) with Plates |
Target Species | Human |
Conjugate | HRP |
Description | Human IFN alpha ELISA Kit (Serum Samples) |
Interassay CV | <8% |
Intraassay CV | <8% |
Gene Symbol | IFNA1,IFNA2 |
Total Assay Time | 3 hr. 15 min. |
Sample Volume | Supernatant, 100 μL |
Storage Requirements | 2°C to 8°C |
Assay Range | 12.5-1000pg/mL |
Test Time | 1 hr. 20 min. |
Regulatory Status | RUO |
Target | Human |
Specificity | No significant cross-reaction with human IFN-ß, IFN-g or IFN-w; mouse or rat IFN-a, IFN-ß, or IFN-g; bovine IFN-t |
Precision | Intra-assay CV ≤ 8%; inter-assay CV ≤ 8% |
For Use With (Equipment) | Colorimetric Microplate Reader |
Product Type | ELISA Kit |
Sample Type | Supernatant |
Label or Dye | HRP |
Invitrogen™ IL-10 Porcine ELISA Kit
A lymphokine involved in immune response
Target Species | Porcine |
---|---|
Conjugate | Biotin |
Interassay CV | 7.2% |
Intraassay CV | 5.1% |
Gene Symbol | CSIF,IL-10 |
Total Assay Time | 3 hr. |
Sample Volume | Plasma, 50 μL; Serum, 50 μL; Supernatant, 100 μL |
Assay Sensitivity | <3 pg/mL |
Storage Requirements | 2°C to 8°C |
Assay Range | 7.8-500 pg/mL |
For Use With (Application) | Colorimetric |
Test Time | 1 hr. 20 min. |
Accession Number | Q29055 |
Regulatory Status | RUO |
Target | Porcine |
Kit Contents | Pre-coated 96 well plate,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
Precision | <3pg/mL |
For Use With (Equipment) | Colorimetric Microplate Reader |
Product Type | ELISA Kit |
Gene ID (Entrez) | 397106 |
Sample Type | Plasma,Serum,Supernatant |
Label or Dye | HRP |
Protein Family | Cytokines & Receptors |
---|---|
Conjugate | Biotin |
Interassay CV | <12% |
Intraassay CV | <10% |
Gene Symbol | IL6 |
Total Assay Time | 4 hr. 45 min. |
Sample Volume | Plasma, 50 μL; Serum, 50 μL; Supernatant, 100 μL |
Assay Sensitivity | 45 pg/mL |
Storage Requirements | 2°C to 8°C |
Research Discipline | Immunology |
For Use With (Application) | ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis |
Test Time | 1 hr. 20 min. |
Detection Method | Colorimetric |
Assay | Solid-phase sandwich ELISA |
Accession Number | P26893 |
Regulatory Status | RUO |
Target | Porcine |
Specificity | The sandwich ELISA antibody pair detects Porcine IL-6. |
Kit Contents | Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers,Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s) and stop solution |
For Use With (Equipment) | Colorimetric Microplate Reader |
Product Type | ELISA Kit |
Gene ID (Entrez) | 399500 |
Sample Type | Plasma,Serum,Supernatant |
Label or Dye | HRP |
Invitrogen™ Porcine TNF-alpha ELISA Kit
Porcine TNF-alpha quantitates porcine TNF-alpha in serum, plasma, supernatant.
Conjugate | Biotin |
---|---|
Interassay CV | <12% |
Intraassay CV | <10% |
Gene Symbol | Tnf |
Total Assay Time | 4 hr 45 min |
Sample Volume | Plasma, 50 μL; Supernatant, 50 μL; Serum, 50 μL |
Assay Sensitivity | 20 pg/mL |
Storage Requirements | 2°C to 8°C |
Assay Range | 20 to 10 ng/mL |
Test Time | 1 hr 20 min |
Accession Number | APC1 protein; Cachectin; C-domain 1; C-domain 2; cTNF; DADB-70P7.1; DIF; ICD1; ICD2; Intracellular domain 1; Intracellular domain 2; N-terminal fragment; NTF; RATTNF; Tnf; TNF alpha; TNF superfamily; TNF a; TNF, macrophage-derived; TNF, monocyte-derived; TNFA; TNF-a; TNFalpha; TNF-alpha; Tnfsf1a; TNFSF2; TNFa; TNLG1F; Tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor alpha (cachetin); tumor necrosis factor alpha precursor; tumor necrosis factor ligand 1 F; Tumor necrosis factor ligand superfamily member 2; Tumor necrosis factor, membrane form; Tumor necrosis factor, soluble form; tumor necrosis factor-alpha; tumor necrosis factor-alpha precursor; tumor-necrosis factor; tumour necrosis factor |
Regulatory Status | Research Use Only |
Target | TNF alpha |
Kit Contents | Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers |
For Use With (Equipment) | Colorimetric Microplate Reader |
Product Type | ELISA |
Gene ID (Entrez) | P23563 |
Sample Type | Plasma,Serum,Supernatant |
Label or Dye | HRP |
Invitrogen™ 2'3'- Cyclic GAMP ELISA Kit
The 2',3'-Cyclic GAMP Competitive ELISA Kit quantitates 2',3'-cyclic GAMP in lysed cells and tissue, tissue culture media, and EDTA plasma.
Protein Family | Hormones & Receptors |
---|---|
Target Species | Mouse |
Conjugate | Biotin |
Interassay CV | <12% |
Intraassay CV | <10% |
Gene Symbol | AV290867,Gha11a1,Prl |
Total Assay Time | 4 hr. 45 min. |
Assay Sensitivity | 30 pg/mL |
Storage Requirements | 2°C to 8°C |
Research Discipline | Endocrinology |
For Use With (Application) | ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis |
Test Time | 1 hr. 20 min. |
Assay | Solid-phase sandwich ELISA |
Accession Number | P06879 |
Regulatory Status | RUO |
Target | Mouse |
Specificity | This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP- 5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, PSelectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF. |
For Use With (Equipment) | Colorimetric Microplate Reader |
Product Type | ELISA Kit |
Gene ID (Entrez) | 19109 |
Sample Type | Plasma,Serum,Supernatant |
Label or Dye | HRP |
Synonym | RP23-351I6.2, AV2908671a1, Prl |