Cell Culture Media
Gibco™ Cell Dissociation Buffer, enzyme-free, PBS
Animal origin-free
Cytiva (Formerly GE Healthcare Life Sciences) SFX-Insect™ Cell Culture Media
Designed to support the growth of multiple insect cell lines and production of a variety of recombinant proteins. HyClone™ SFX Insect™ Cell Culture Media is a protein-free media that support cell culture growth.
Gibco™ Ham's F-12K (Kaighn's) Medium
A modification of Ham's F-12 Nutrient Mixture
Gibco™ DMEM, no glucose
Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ DMEM, No Glucose is used in cell culture processing applications.
Gibco™ RPMI 1640 Medium, no glutamine, no phenol red
Animal origin-free
Cytiva (Formerly GE Healthcare Life Sciences) HyClone™ Dulbecco's Modified Eagles Medium
Designed to support cell culture growth and biomanufacturing processes.
Corning™ cmRL 1066
For use in both serum supplemented and unsupplemented culture
Gibco™ Essential 8™ Flex Medium Kit
Essential 8™ Flex is a xeno-free and feeder-free medium that supports the culture of pluripotent stem cells (PSCs) without the need for weekend feeding. Gibco™ Essential 8™ Flex Medium Kit is provided in a convenient two-component kit (500mL basal medium and 10mL supplement).
Corning™ RPMI 1640 Medium (Mod.) 1X with L-Glutamine
Guaranteed low endotoxin levels ≤0.025EU/mL
Gibco™ DMEM, high glucose, pyruvate
Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ DMEM, High Glucose, Pyruvate is used in cell culture processing applications.
Gibco™ RPMI 1640 Medium, no folic acid
Originally developed to culture human leukemic cells in suspension and as a monolayer
Gibco™ StemPro™ MSC SFM
StemPro™ MSC SFM
Gibco™ MEM α, nucleosides, no phenol red
Minimum Essential Medium is widely used for mammalian cell culture as well as selection for transfected DHFR negative cells
Gibco™ Recovery™ Cell Culture Freezing Medium
Complete, ready-to-use freezing medium for cryopreservation of a wide variety of mammalian cells, including CHO-S, CHO-K1, HEK 293, Jurkat, and NIH 3T3
Corning™ cellgro™ DMEM Liquid Classical Cell Culture Media
Products undergo a variety of tests to determine functionality and toxicity using growth-promotion analysis. Corning™ cellgro™ DMEM Liquid Classical Cell Culture Media support cell culture growth and subsititution of Pyridoxine HCl for Pyridoxal ensures improved stability over original formulation.
Gibco™ Dispase II, powder
Amino-endo peptidase that hydrolyzes N-terminal peptide bonds of non-polar amino acid residues
Gibco™ Essential 8™ Medium
Essential 8™ Medium
Gibco™ DMEM, high glucose, no glutamine, no phenol red
Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ DMEM, High Glucose, No Glutamine, No Phenol Red is used in cell culture processing applications.
Corning™ Poloxamer 188, 10% Solution
Acts as surfactant used in cell culture to protect cells in suspension against possible damage during transfer, freezing and thawing, and stirring.
Gibco™ DMEM/F-12
Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ DMEM/F-12 is used in cell culture processing applications.
Gibco™ Neurobasal™ Medium
Used in the growth of neuronal cells from hippocampus, cortex and otregions of the brain. Gibco™ Neurobasal™ Medium is a basal medium that meets the special cell culture requirements of pre-natal and embryonic neuronal cells when used with Gibco™ B-27™ Supplement.
Cytiva (Formerly GE Healthcare Life Sciences) HyClone™ SFM4Insect™ Media
Support growth of many key insect cell lines and production of a variety of recombinant proteins. HyClone™ SFM4Insect™ Media is free of animal-derived components (ADCF).
Gibco™ Opti-MEM™ I Reduced Serum Medium
Opti-MEM™ I Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change in growth rate or morphology.
Corning™ Basal Cell Culture Liquid Media - Alpha Modification of Eagle's Medium 1X (AMEM)
Contains Earle's salts, ribonucleosides, deoxyribonucleosides, and L-glutamine.
Cytiva (Formerly GE Healthcare Life Sciences) HyClone™ RPMI 1640 Media
Designed to support cell culture growth and biomanufacturing processes.
Lonza™ BioWhittaker™ 293 Cell Serum-Free Media
Completely balanced to support vaccine and viral vector production of high-density human embryonic kidney (HEK) 293 cell culture environments
Gibco™ IMDM
Iscove's Modified Dulbecco's Medium is well suited for rapidly proliferating, high-density cell cultures, including Jurkat, COS-7, and macrophage cells
Gibco™ RPMI 1640 Medium, no glucose
Originally developed to culture human leukemic cells in suspension and as a monolayer
Cell culture media support the growth and maintenance of a variety of mammalian and other cell lines in vitro, as either anchorage-dependent or suspension cultures. Cell culture offers convenience, control, consistency and reproducibility of results, especially when using clonal cells.
Cell culture has multiple applications in cellular and molecular biology:
- Studying normal cell physiology and biochemistry
- Industrial bioprocess/bioreactor manufacturing of vaccines, therapeutic proteins and monoclonal antibodies using recombinant, high-yield protein expression systems
- Pharmaceutical screening and development, including drug metabolism and toxicology
- Other emerging applications in stem cell culture, immunotherapy and cell therapy
Commercially available cell culture media are subject to very stringent testing and manufacturing controls. They fall into several categories:
- Classical mammalian cell culture media (DMEM, MEM, RPMI 1640, McCoy’s, Ham’s, BME and EBSS) contain all the nutrients that cells need, such as amino acids, minerals, vitamins and glucose
- Individual cell culture reagents and supplements, including growth factors (e.g. pyruvate), antibiotics, hormones, cryopreservation media, cell dissociation reagents and animal-derived sera (e.g., FBS foetal bovine serum) which contain a ready-made natural mixture of nutrients
- Speciality media, including stem cell culture media, transfection media, and serum-free or animal-derived component-free (ACDF) additives
- Ancillary buffers and process liquids, including balanced salt solutions (PBS phosphate buffered saline, Hank’s HBSS, Earle’s EBBS), sodium bicarbonate and HEPES buffers, phenol red, cell culture grade water, and adherent cell microcarriers
Cell culture media are typically supplied as presterilised ready-to-use liquids, concentrates, powders or other solids in bottles, or else as single-use sealed bags for integration into compatible cell culture harvesting systems.
Commercially available cell culture media are subject to very stringent testing and manufacturing controls. They fall into several categories:
Commercially available cell culture media are subject to very stringent testing and manufacturing controls. They fall into several categories:
Commercially available cell culture media are subject to very stringent testing and manufacturing controls. They fall into several categories: