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Applied Biosystems™ AgPath-ID™ One-Step RT-PCR Reagents
AgPath-ID™ One-Step RT-PCR Reagents are designed for sensitive, robust amplification of RNA targets using a rapid single-tube TaqMan™ real-time reverse transcription PCR (RT-PCR) strategy.
| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | 25 X |
| Description | Works on all AB platforms, but is optimized for the 7500. |
| Reverse Transcriptase | ArrayScript™ |
| Concentration | 25X |
| PCR Method | 1-step RT-qPCR |
| Detection Method | Primer-probe |
| Polymerase | AmpliTaq Gold DNA Polymerase |
| Format | Tube |
| Product Type | One-Step RT-PCR Reagent |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | 7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex, QuantStudio™ Absolute Q Digital PCR System |
| Sample Type | RNA |
| Reaction Speed | Standard |
| Product Line | AgPath-ID, Ambion |
Invitrogen™ Zero Blunt™ PCR Cloning Kit, without competent cells
Offers an easy method for high-efficiency (>80%) cloning of blunt-end PCR products amplified with proofreading, thermostable DNA polymerases
| Promoter | T7 |
|---|---|
| Content And Storage | Zero Blunt™ PCR cloning kits contain linearized pCR™-Blunt vector, ExpressLink™ T4 DNA ligase , 5X ExpressLink™ T4 DNA ligase buffer, control template, dNTPs, sterile water, and M13 forward and reverse primers. Store all components at -20°C. All reagents are guaranteed stable for 6 months when properly stored. |
| Format | Kit |
| Product Type | PCR Cloning Kit |
| Cloning Method | Blunt PCR |
| Bacterial or Yeast Strain | Not Included |
| Vector | Blunt DNA Cloning Vectors |
| Product Line | TOPO, Zero Blunt |
Applied Biosystems™ PrepMan™ Ultra Sample Preparation Reagent
The PrepMan™ Ultra Sample Preparation Reagent was developed for the rapid preparation of DNA template from Gram-negative food-borne pathogens for use in PCR amplification reactions.
Applied Biosystems™ AgPath-ID™ One-Step RT-PCR Reagents with manual
AgPath-ID™ One-Step RT-PCR Reagents are designed for sensitive, robust amplification of RNA targets using a rapid single-tube TaqMan™ real-time reverse transcription PCR (RT-PCR) strategy.
| GC-Rich PCR Performance | High |
|---|---|
| Fidelity (vs. Taq) | 25 X |
| Description | Works on all AB platforms, but is optimized for the 7500. |
| Reverse Transcriptase | ArrayScript™ |
| Concentration | 25X |
| PCR Method | 1-step RT-qPCR |
| Detection Method | Primer-probe |
| Polymerase | AmpliTaq Gold DNA Polymerase |
| Format | Tube |
| Product Type | One-Step RT-PCR Reagent |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | 7500 System |
| Reaction Speed | Standard |
| Product Line | AgPath-ID, Ambion |
Applied Biosystems™ ExoSAP-IT™ Express PCR Product Cleanup Reagent
Removes excess primers and dNTPs. Does not interfere with downstream applications.
| Content And Storage | -20°C |
|---|
Applied Biosystems™ HT ExoSAP-IT™ Fast High-Throughput PCR Product Cleanup Reagent
Removes excess primers and dNTPs. Does not interfere with downstream applications. Ideal for high-throughput PCR product cleanup using liquid handling platforms and multi-channel pipettes.
| Content And Storage | Store at −20°C. Stable for one month at 4°C |
|---|
Applied Biosystems™ TaqMan™ RNase P Detection Reagents Kit
TaqMan™ RNase P Detection Reagents Kit
Applied Biosystems™ ExoSAP-IT™ PCR Product Cleanup Reagent
ExoSAP-IT™ PCR Product Cleanup Reagent is used for enzymatic cleanup of amplified PCR product by hydrolyzing excess primers and nucleotides in a single step
Invitrogen™ SuperScript™ III First-Strand Synthesis SuperMix for qRT-PCR
SuperScript III First-Strand Synthesis SuperMix for qRT-PCR provides the high-temperature capability of SuperScript III Reverse Transcriptase in an optimized SuperMix format for the synthesis of first-strand cDNA for use in real-time quantitative RT-PCR (qRT-PCR).
| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Format | Super Mix |
| Reverse Transcriptase | SuperScript III |
| Reaction Format | Master Mix |
| Final Product Type | First-Strand cDNA |
| Reaction Speed | 30 min. |
| Reagent Type | Reverse Transcription |
| Optimal Reaction Temperature | 50°C |
| Technique | Reverse Transcription |
| Starting Material | RNA |
| For Use With (Application) | Real Time PCR (qPCR) |
| Detection Method | qPCR |
Invitrogen™ RNAsecure™ RNase Inactivation Reagent
Invitrogen™ RNAsecure™ (patents pending) is a unique non-enzymatic reagent that will irreversibly inactivate RNases in solution.
| Shipping Condition | Dry Ice |
|---|---|
| Content And Storage | Store RNAsecure™ at -20°C. |
| Form | Liquid |
| Product Type | RNase Control Reagent |
| Purity | Molecular Biology Grade |
| For Use With (Application) | RT-PCR, In Vitro Transcription |
| Product Line | Ambion, RNAsecure |
Applied Biosystems™ ExoSAP-IT™ PCR Product Cleanup Reagent
ExoSAP-IT™ PCR Product Clean-up Reagent is used for enzymatic clean-up of amplified PCR product. It hydrolyses excess primers and nucleotides in a single step.
| Shipping Condition | Dry Ice |
|---|---|
| Content And Storage | Shipped on dry ice. Store at -20°C. |
| Protein Form | Recombinant |
| Sample Type | PCR Products |
| Research Category | Genetic Diseases |
| For Use With (Application) | PCR Purification |
| Product Line | ExoSAP-IT |
Applied Biosystems™ TaqMan™ Sample-to-SNP™ Kit (200mL Sample prep, 10mL PCR)
Go from Biological Sample to Genotyping Results in less than one hour.
Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit, with One Shot™ TOP10 Chemically Competent E. coli cells
Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of blunt-ended PCR products amplified with proofreading thermostable polymerases into a plasmid vector
| Promoter | SP6 |
|---|---|
| Content And Storage | Box 1: • Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. Box 2: • One Shot™ Chemically Competent or Electrocomp™ E. coli • S.O.C. medium • Supercoiled pUC19 control plasmid Store at -68 to -85°C. |
| Format | Kit |
| Product Type | PCR Cloning Kit |
| Cloning Method | Blunt TOPO™ |
| Bacterial or Yeast Strain | TOP10 |
| Cell Type | Chemically Competent |
Invitrogen™ DNAZap™ PCR DNA Degradation Solutions
DNAZap™ Solutions are two solutions that are innocuous when used alone, but become a potent nucleic acid degrading reagent when mixed.
Invitrogen™ TRI Reagent™ Solution
Tri Reagent™ solution is a complete, ready-to-use reagent for the isolation of total RNA or the simultaneous isolation of RNA, DNA, and protein from a variety of biological samples.