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Thermo Scientific™ DH10B Competent Cells; High Efficiency
High efficiency, chemically competent E. coli cells. The DH10B strain is suitable for cloning DNA containing methylcytosine, 5-hydroxymethylcytosine, and methyladenine, allowing both prokaryotic and eukaryotic genomic DNA to be cloned efficiently.
Thermo Scientific™ pBR322 Plasmid DNA
Optimize cloning with Thermo Scientific™ pBR322 DNA, a 4361 bp double-stranded closed circular medium copy plasmid DNA.
Invitrogen™ One Shot™ TOP10 Electrocomp™ E. coli
One Shot TOP10 Electrocomp E. coli cells are ideal for high-efficiency cloning and plasmid propagation. Provided at a transformation efficiency of >1 x 1010 cfu/ μg supercoiled DNA, these cells allow for stable replication of high copy number plasmids.
| Shipping Condition | Dry Ice |
|---|---|
| Blue/White Screening | Yes (lacZΔM15) |
| Cloning Unstable DNA | No |
| Cloning Methylated DNA | Yes (mcrA) |
| Transformation Efficiency Level | High Efficiency (>1 x 1010 cfu/μg) |
| High-throughput Compatibility | Low |
| Improves Plasmid Quality | Yes (endA1) |
| Preparing Unmethylated DNA | No |
| Plasmid | May be used for plasmids >20 kb |
| Reduces Recombination | Yes (recA13) |
| T1 Phage - Resistant (tonA) | No |
| Antibiotic Resistance Bacterial | Yes (Streptomycin) |
| Format | Tube |
| Product Type | Electrocompetent Cells |
| Species | E. coli (K12) |
| Product Line | One Shot™ |
| Contains F' Episome | No |
Invitrogen™ MAX Efficiency™ Stbl2™ Competent Cells
MAX Efficiency Stbl2 Competent Cells are high-efficiency chemically competent cells specifically designed for cloning unstable inserts.
Invitrogen™ ElectroMAX™ Stbl4™ Competent Cells
ElectroMAX Stbl4 Competent Cells are specifically designed for cloning unstable inserts. This strain is a derivative of Stbl2 cells (engineered from JM109/J5 strain).
Invitrogen™ One Shot™ BL21(DE3)pLysS Chemically Competent E. coli
One Shot BL21(DE3)pLysS Chemically Competent E. coli cells are ideal for use with bacteriophage T7 promoter-based expression systems such as pRSET and pET.
| Shipping Condition | Dry Ice |
|---|---|
| Blue/White Screening | No |
| Cloning Methylated DNA | No |
| Transformation Efficiency Level | Medium Efficiency (1 x 108 to 1 x 109 cfu/μg) |
| ToxicProteins | No |
| High-throughput Compatibility | Low |
| Improves Plasmid Quality | No |
| ImprovesProteinStability | Yes (lon, ompT) |
| Promoter | T7 |
| Preparing Unmethylated DNA | Yes (dcm) |
| Reduces Recombination | No |
| ImprovesRNAStability | Yes (pLysS) |
| T1 Phage - Resistant (tonA) | No |
| Antibiotic Resistance Bacterial | Yes (Chloramphenicol) |
| Format | Tube |
| Product Type | Chemically Competent Cells |
| Species | E. coli (B) |
| Product Line | One Shot™ |
| Contains F' Episome | No |
Invitrogen™ One Shot™ BL21-AI™ Chemically Competent E. coli
One Shot BL21-AI Chemically Competent E. coli cells are designed for applications that require tight regulation and strong expression of toxic proteins from any T7-promoter-based expression systems.
Invitrogen™ MegaX DH10B T1R Electrocomp™ Cells
MegaX DH10B T1R Electrocomp Cells are the highest-efficiency electrocompetent cells available. Improved production processes provide a transformation efficiency reaching >3 x 1010 cfu/μg of pUC DNA.
Invitrogen™ One Shot™ Mach1™ T1 Phage-Resistant Chemically Competent E. coli
One Shot Mach1 T1 Phage-Resistant Chemically Competent E. coli are the fastest growing chemically competent cells with a doubling time approximately 50 minutes.
Invitrogen™ TOPO™ TA Cloning™ Kit for Sequencing, with One Shot™ TOP10 Chemically Competent E. coli
Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for sequencing
| Format | Kit |
|---|---|
| Product Type | Cloning Kit |
| Cloning Method | TOPO™-TA |
| Bacterial or Yeast Strain | TOP10 |
| Vector | TOPO-TA Cloning Vectors |
| Product Line | One Shot™ |
Invitrogen™ Phospha-Light™ SEAP Reporter Gene Assay System
Chemiluminescent reporter gene assay for sensitive detection of secreted human placental alkaline phosphatase used as a reporter protein.
| Content And Storage | This kit contains the substrate, required buffers, enhancer, and control Placental Alkaline Phosphatase. Store the product at 4°C. |
|---|---|
| Form | Liquid |
| Readout | End Point |
| Compatible Cells | Mammalian Cells |
| Target Enzyme | Alkaline Phosphatase |
| Substrate Type | AP (Alkaline Phosphatase) Substrate |
| For Use With (Application) | Enzyme Assay |
| High-throughput Compatibility | High-throughput Compatible |
| Detection Method | Chemiluminescence |
| Substrate | CSPD™ |
| Assay | Reporter Assay |
| Substrate Properties | Chemical Substrate |
| For Use With (Equipment) | Luminometer (Microplate) |
| Product Type | Secreted Human Placental Alkaline Phosphatase Reporter Gene Assay System |
| Sample Type | Cell Cultures |
| Label or Dye | Unconjugated |
| Product Line | NovaBright™, Phospha-Light™ |
| Shipping Condition | Approved for shipment at Room Temperature or on Wet or Dry Ice |
|---|---|
| Content And Storage | Store in freezer (-5 to -30°C). |
| Form | Lyophilized Powder |
| Product Type | Yeast tRNA |
| Reagent Type | Yeast tRNA |
| For Use With (Application) | Carrier for Nucleic Acid Precipitation |
Invitrogen™ One Shot™ MAX Efficiency™ DH5α-T1R Competent Cells
One Shot MAX Efficiency DH5α-T1R Competent Cells are derived from the popular DH5α strain and carry the tonA (fhuA) mutation that confers resistance to T1 and T5 phage.
Invitrogen™ MAX Efficiency™ DH10Bac Competent Cells
Capable of supporting site-specific recombination between pFastBac™ and bMON14272
Invitrogen™ MAX Efficiency™ DH10B Competent Cells
MAX Efficiency DH10B Competent Cells are highly efficient E. coli suitable for a variety of molecular biology applications. This strain is one of the most commonly used strains for everyday applications due to key strain features such as high transformation efficiency.