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Invitrogen™ TURBO DNA-free™ Kit

Ideal tool for clearing residual DNA

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Modifying Enzymes

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Applied Biosystems™ RNase Inhibitor

No DNase or endonuclease activity

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RNA Preparation and Purification

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Thermo Scientific™ CloneJET PCR Cloning Kit

Efficiently clone PCR products generated with any thermostable DNA polymerase using Thermo Scientific™ CloneJET™ PCR Cloning Kit, a positive selection system.

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Specifications

PCR Cloning Kits

Antibiotic Resistance Bacterial	Ampicillin (AmpR)
Format	Kit
Sample Type	PCR Products
Cloning Method	Restriction Enzyme⁄ MCS, Blunt PCR
For Use With (Application)	PCR Cloning
Vector	pJET1.2/blunt
Product Line	CloneJET

Thermo Scientific™ FastAP Thermosensitive Alkaline Phosphatase (1 U/μL)

Catalyze the removal of 5'- and 3'-phosphate groups from DNA, RNA, nucleotides, and proteins using this thermosensitive alkaline phosphatase .

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Specifications

Modifying Enzymes

Product Type	Alkaline Phosphatase
Enzyme	Phosphatase
Compatible Buffer	PCR Buffer, Enzyme Buffer
Concentration	1 U/μL

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Thermo Scientific™ FastDigest BglII

Cut at A^GATCT sites with FastDigest™ BglII restriction enzyme, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer.

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Specifications

Restriction Enzymes

Sensitive to Heat Inactivation	No
Product Type	Restriction Enzyme
Enzyme	Bgl II
Methylation Sensitivity	Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Research Category	Traditional Cloning
Compatible Buffer	10X FastDigest Buffer/FastDigest Green Buffer
Overhang	5' protruding ends
Type IIS RE	No
Optimal Reaction Temperature	37°C
Product Line	FastDigest™

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Thermo Scientific™ FastDigest LguI (IIs class)

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.

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Restriction Enzymes

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Thermo Scientific™ pUC18 DNA

Optimize cloning with this double-stranded closed circular high copy plasmid DNA, 2686 base pairs with a molecular weight of 1.74 x 10⁶ Da isolated from E.coli.

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DNA Cloning and Mapping and Mutagenesis

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Invitrogen™ MAX Efficiency™ Stbl2™ Competent Cells

MAX Efficiency Stbl2 Competent Cells are high-efficiency chemically competent cells specifically designed for cloning unstable inserts.

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Competent Cells

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Thermo Scientific™ phi29 DNA Polymerase (10 U/μL)

Thermo Scientific phi29 DNA Polymerase is a highly processive polymerase featuring strong strand displacement activity, which allows for highly efficient isothermal DNA amplification.

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Specifications

Isothermal Amplification Reagents

Shipping Condition	Dry Ice
Polymerase	phi29 DNA Polymerase
Product Type	Stand-alone enzyme
Reaction Time	4 hours
Concentration	10 U/μL
Optimal Reaction Temperature	30°C
For Use With (Application)	MDA-WGA,RCA

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Applied Biosystems™ TaqPath™ BactoPure™ Microbial Detection Master Mix

TaqPath BactoPure Microbial Detection Master Mix is optimized for rapid low-level microbial detection even in the presence of PCR inhibitors.

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Specifications

Real Time PCR Master Mixes

Content And Storage	Contains 2X TaqPath BactoPure Microbial Detection Master Mix that includes: • Hot-start AmpliTaq Fast DNA Polymerase • Heat-labile uracil-DNA glycosylase (UNG) • dNTPs (with dUTP) • ROX dye (passive reference) • Optimized buffer Store at 2°C to 8°C in the dark.
InhibitorToleranceSimple	Very High
For Use With (Application)	Microbial Detection
PCR Method	qPCR
Multiplex Capability	Up to 3-plex
Detection Method	Primer-probe
Includes	dUTP, UNG/UDG
Thermostability	72 hr., Assembled Reaction
Sensitivity	Down to 1 copy
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio12k Flex Dx, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio 5 Dx, QuantStudio 6 Pro, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 7 Pro, QuantStudio 7 Pro Dx, ViiA™ 7 System, ViiA 7 Dx, QuantStudio™ Dx, StepOnePlus™ System, 7500 Fast Dx System, StepOne™ System
Sample Type	DNA
Reaction Speed	Fast or Standard
Target Specificity	cDNA or gDNA only
Product Line	TaqPath™

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Invitrogen™ RNAqueous™ Total RNA Isolation Kit

The RNAqueous Total RNA Isolation Kit is for phenol-free total RNA isolation from 1–75 mg of tissue or from 10^2–10^7 cells, using a guanidinium-based lysis/denaturant and glass fiber filter separation technology.

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Specifications

RNA Isolation and Purification Products

Shipping Condition	Room Temperature
For Use With (Application)	RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling

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Applied Biosystems™ PowerTrack™ SYBR Green Master Mix for qPCR

PowerTrack SYBR Green Master Mix is a pre-formulated, optimized, universal 2X master mix for real-time PCR workflows with built-in visual dye–based indicators for accurate reaction set up.

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Specifications

Real Time PCR Master Mixes

Shipping Condition	Dry Ice
Content And Storage	• PowerTrack SYBR Green Master Mix • 40X Yellow Sample Buffer Store at -20±5°C in the dark.
InhibitorToleranceSimple	High
Concentration	2X
For Use With (Application)	Gene Expression,Microbial Detection
PCR Method	qPCR
Multiplex Capability	Single-plex
Detection Method	SYBR
Includes	dUTP, UNG/UDG
Thermostability	8 hr., Assembled Reaction
Polymerase	Taq DNA Polymerase
Format	Tube
Product Type	Real Time PCR SYBR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Flex, QuantStudio 7 Pro, QuantStudio 12K Flex, Bio-Rad CFX Connect, Bio-Rad CFX384 Touch, Bio-Rad CFX96 Touch, BioRad CFX96, BioRad iCycler iQ, BioRad iQ5, BioRad MyiQ, ViiA 7 System, StepOne System, MJ Chromo4, MJ Opticon, StepOnePlus System, Roche LightCycler 480, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000
Sample Type	DNA
Reaction Speed	Fast or Standard
Target Specificity	cDNA or gDNA only
Product Line	Applied Biosystems™

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Thermo Scientific™ dNTP Mix (25 mM each)

Thermo Scientific dNTP Mix (25 mM) contains premixed aqueous solutions of dATP, dCTP, dGTP and dTTP, each at a final concentration of 25 mM.

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Specifications

dNTPs

Shipping Condition	Dry Ice
Purity or Quality Grade	>99%
Form	Liquid
Label or Dye	Unlabeled
Concentration	25 mM
For Use With (Application)	Standard PCR,High-fidelity PCR,RT-PCR,Real Time PCR (qPCR)

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TaqMan™ Control Genomic DNA (human)

Conveniently packaged in a PCR-ready concentration for use as a control template in PCR reactions

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Nucleic Acid Controls

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Gibco™ ExpiFectamine™ Sf Transfection Reagent

Gibco™ ExpiSf™ Transfection Reagant is a cationic lipid-based reagent for efficient DNA transfection of insect cells using fast, flexible protocols.

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Transfection Reagents

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