Filtered Search Results
Invitrogen™ PureLink™ Quick Plasmid Miniprep Kit
The PureLink Quick Plasmid Miniprep Kit is designed for rapid isolation of sequencing-grade plasmid DNA from E.coli in about 30 minutes, using silica membrane columns.
Shipping Condition | Room Temperature |
---|---|
Content And Storage | • Resuspension buffer • RNase A (20 mg/mL) • Lysis buffer • Precipitation buffer • Wash buffers • TE buffer • Spin columns in wash tubes • Elution tubes |
Final Product Type | Plasmid DNA |
Isolation Technology | Silica Spin Column |
Column Type | Spin Column |
Concentration | 20 mg/mL |
High-throughput Compatibility | Not High-throughput Compatible (Manual) |
For Use With (Application) | Next-Generation Sequencing, Cloning, Sequencing, Nucleic Acid Labeling, PCR, Transformation |
Test Time | 30 to 45 min. |
Prep Scale | < 100 μg (Small-Scale) Plasmid DNA |
Target | Plasmid DNA |
Format | Spin Column |
Product Type | Quick Plasmid MiniPrep Kit |
Sample Type | Bacterial Culture |
Product Line | PureLink™ |
Applied Biosystems™ SYBR™ Green Universal Master Mix
Everything needed for SYBR™ Green dye-based PCR amplification and detection in a convenient, single-tube format.
Polymerase | AmpliTaq Gold DNA Polymerase |
---|---|
GC-Rich PCR Performance | High |
Format | Tube |
Product Type | Real Time PCR SYBR Master Mix |
Passive Reference Dye | ROX (Pre-mixed) |
Reaction Speed | Standard |
Label or Dye | SYBR Green |
Concentration | 2X |
For Use With (Application) | Gene Expression |
PCR Method | qPCR |
Product Line | SYBR™ |
Detection Method | SYBR |
Applied Biosystems™ GeneAmp™ dNTP Blend (2.5 mM ea)
GeneAmp dNTPs are deoxynucleotide triphosphates dissolved in glass-distilled water and titrated to pH 7.0 with NaOH. Each vial consists of 1 mL of 2.5 mM each of dATP, dCTP, dGTP, and dTTP, yielding a 10 mM blend of dNTPs.
Shipping Condition | Dry Ice |
---|---|
Form | Liquid |
Concentration | 10 mM |
For Use With (Application) | Standard PCR |
Product Line | GeneAmp™ |
Applied Biosystems™ TaqMan™ Universal PCR Master Mix, no AmpErase™ UNG
TaqMan™ Universal PCR Master Mix, No AmpErase™ UNG is the ideal reagent solution when you need a Master Mix for multiple 5' nuclease DNA applications.
Shipping Condition | Wet Ice |
---|---|
Polymerase | AmpliTaq Gold DNA Polymerase |
GC-Rich PCR Performance | High |
Product Type | Universal PCR Master Mix |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, GeneAmp 9600, StepOne™, Standard Mode, StepOnePlus™, Standard Mode |
Sample Type | DNA (Genomic), cDNA |
Reaction Speed | Standard |
For Use With (Application) | Gene Expression,Genotyping,miRNA analysis |
PCR Method | qPCR |
Product Line | TaqMan™ |
Detection Method | Primer-probe |
Invitrogen™ Fixative-Free Lysing Solution, High-Yield Lyse
Fixative-Free Lysing Solution, High-Yield Lyse
Invitrogen™ Qubit™ 1X dsDNA High Sensitivity (HS) and Broad Range (BR) Assay Kits
Highly selective for dsDNA over RNA, the Qubit 1X dsDNA HS and BR Assay Kits offer easy-to-use formulations for the accurate quantitation of dsDNA in 100 and 500 assay formats. Lambda Standards provide sufficient material for up to 500 samples.
Green Features | Fewer resources used, Less waste |
---|---|
Product Line | Qubit |
Invitrogen™ TRIzol™ Reagent
TRIzol™ Reagent is a complete, ready-to-use reagent for the isolation of high-quality total RNA or the simultaneous isolation of RNA, DNA, and protein from a variety of biological samples.
Shipping Condition | Room Temperature |
---|---|
Final Product Type | Total RNA, DNA, Protein |
Sample Type | Bacteria,Cells,Plants,Tissue,Yeast |
Isolation Technology | Organic Extraction |
Starting Material Amount | Bacteria: ≤107 cells Cells: ≤107 Plant: ≤107 cells, 100 mg Tissue: ≤100 mg Yeast: ≤107 cells |
Purification Time | 1 hr. |
Yield | DNA: ≤7 μg RNA: ≤73 μg Protein: varies |
Elution Volume | 20 to 600 μL |
For Use With (Application) | RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern/Southern/Western blotting, in vitro translation, nuclease protection assays, nucleic acid labeling, hybridization, enzymatic assays, immunoprecipitation, gel shift assays, 2D gel electrophoresis |
High-throughput Compatibility | Not High-throughput Compatible (Manual) |
Invitrogen™ RNaseOUT™ Recombinant Ribonuclease Inhibitor
Protects mRNA and improves total cDNA yields
Invitrogen™ Qubit™ dsDNA Quantification Assay Kits
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
For highly selective detection of dsDNA over RNA, even in the presence of contaminants. The Qubit dsDNA Assay Kits are accurate from 10 pg/uL to 100 ng/uL, and 100 pg/uL to 1,000 ng/uL, respectively.
Shipping Condition | Room Temperature |
---|---|
Excitation/Emission | 510/527 |
For Use With (Equipment) | Qubit Fluorometer |
Product Line | Qubit |
Detection Method | Fluorescence |
Invitrogen™ Lipofectamine™ 2000 Transfection Reagent
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
Versatile transfection reagent that has been shown to effectively transfect the widest variety of adherent and suspension cell lines
Applied Biosystems™ High-Capacity cDNA Reverse Transcription Kit
The High Capacity cDNA Reverse Transcription Kit, available with or without RNase Inhibitor, contains all components necessary for the quantitative conversion of up to 2 μg of total RNA into single-stranded cDNA in a single 20 μL reaction.
Shipping Condition | Dry Ice |
---|---|
Format | Kit |
Reverse Transcriptase | MultiScribe |
Reaction Format | Separate components |
Final Product Type | First-Strand cDNA |
Reaction Speed | 120 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Yes |
Concentration | 50 U/μL |
Technique | Reverse Transcription |
Starting Material | RNA |
Thermo Scientific™ GeneJET Plasmid Miniprep Kit
Thermo Scientific GeneJET Plasmid Miniprep Kit utilizes an exclusive silica-based membrane technology in the form of a convenient spin column.
Target | Plasmid DNA |
---|---|
Format | Spin Column |
Product Type | Plasmid MiniPrep Kit |
Sample Type | Bacteria |
Isolation Technology | Silica Column |
Column Type | Spin Column |
Scale | Mini |
High-throughput Compatibility | Compatible (Vacuum manifold) |
For Use With (Application) | PCR, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, In Vitro Transcription |
Test Time | <14 min |
Product Line | GeneJET |
Prep Scale | < 100 μg (Small-Scale) Plasmid DNA |
Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerase (2 U/μL)
Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR.
Shipping Condition | Dry Ice |
---|---|
GC-Rich PCR Performance | High |
Content And Storage | • Phusion DNA Polymerase (2 U/μL) • 5X Phusion HF & GC Buffers • DMSO • 50 mM MgCl2 solution Phusion HF Buffer and Phusion GC Buffers each provide 1.5 mM MgCl2 in the final 1X concentration. Store at -20°C. |
Fidelity (vs. Taq) | 52X |
Size (Final Product) | 20 kb or less |
Reaction Format | Standalone |
Product Type | High-Fidelity DNA Polymerase |
Reaction Speed | Fast |
Hot Start | No |
Overhang | Blunt |
Concentration | 2 U/μL |
For Use With (Application) | Standard PCR,High-fidelity PCR |
Invitrogen™ Lipofectamine™ RNAiMAX Transfection Reagent
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
Provides highest transfection efficiencies on widest variety of cell types for siRNA-mediated gene knockdown experiments
Shipping Condition | Approved for shipment at Room Temperature or on Wet Ice |
---|---|
Format | 6-well Plate, 12-well Plate, 24-well Plate, 48-well Plate, 96-well Plate, Flasks |
Product Type | Transfection Reagent |
Transfection Technique | Lipid-based Transfection |
Serum Compatible | Yes |
Green Features | Sustainable packaging |
High-throughput Compatibility | High-throughput Compatible |
For Use With (Application) | Transfection |
Product Line | Lipofectamine™ |
Cell Type | Established Cell Lines,Stem Cells,Primary Cells,Hard-to-Transfect Cells |
Invitrogen™ SuperScript™ III Reverse Transcriptase
Invitrogen SuperScript III Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) created by the introduction of several mutations for reduced RNase H activity, increased half-life, and improved thermal stability.
Shipping Condition | Dry Ice |
---|---|
GC-Rich PCR Performance | High |
Reverse Transcriptase | SuperScript III |
Final Product Type | First-Strand cDNA |
Concentration | 200 U/μL |
Format | Stand-alone Enzyme |
Size (Final Product) | Up to 12.3 kb |
Reaction Format | Separate components |
Reaction Speed | 30 to 50 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Reduced |
Optimal Reaction Temperature | 50°C |
Technique | Reverse Transcription |
Starting Material | RNA |