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Thermo Scientific™ CloneJET PCR Cloning Kit
Efficiently clone PCR products generated with any thermostable DNA polymerase using Thermo Scientific™ CloneJET™ PCR Cloning Kit, a positive selection system.
Antibiotic Resistance Bacterial | Ampicillin (AmpR) |
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Format | Kit |
Sample Type | PCR Products |
Cloning Method | Restriction Enzyme⁄ MCS, Blunt PCR |
For Use With (Application) | PCR Cloning |
Vector | pJET1.2/blunt |
Product Line | CloneJET |
Thermo Scientific™ Lambda DNA
Generate DNA size marker fragments with this linear, double-stranded DNA, a common substrate for restriction endonucleases.
Invitrogen™ GeneArt™ Gibson Assembly HiFi Master Mix
GeneArt Gibson Assembly HiFi Master Mix enables DNA assembly and cloning via a technique that allows multiple overlapping DNA fragments to be seamlessly linked in a one-step, 15–60 minute isothermal reaction.
Shipping Condition | Dry Ice |
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Content And Storage | Includes master mix, positive control, and water and accommodates the use of your own competent cells. |
Workflow Step | DNA Assembly |
Number of Fragments | Up to 6 fragments |
Product Type | HiFi Master Mix |
Control Type | Positive Control |
Cloning Method | Seamless cloning |
For Use With (Application) | Seamless Cloning & Genetic Assembly |
Product Line | GENEART™ |
Thermo Scientific™ Rapid DNA Ligation Kit
Enable fast sticky-end or blunt-end DNA ligation in only 5 minutes at room temperature.
Product Type | Rapid DNA Ligation Kit |
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Enzyme | DNA Ligase |
Thermo Scientific™ pBR322 Plasmid DNA
Optimize cloning with Thermo Scientific™ pBR322 DNA, a 4361 bp double-stranded closed circular medium copy plasmid DNA.
Invitrogen™ Second Strand cDNA Synthesis Kit
Invitrogen™ Second Strand cDNA Synthesis Kit is design to produce double-stranded cDNA using first strand reaction without the need for intermediate organic extraction or ethanol precipitation steps. This convenient single-tube format speeds up the synthesis procedure and maximizes cDNA recovery. The kit contains premixed components to reduce the number of pipetting steps necessary to complete the procedure.
Reaction Format | Separate Components |
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Final Product Type | Double Stranded cDNA |
Product Type | cDNA Synthesis Kit |
For Use With (Application) | Cloning |
Thermo Scientific™ pUC19 DNA
Optimize cloning with this double-stranded closed circular high copy plasmid DNA, 2686 base pairs with a molecular weight of 1.74 x 106 Da isolated from E.coli.
Applied Biosystems™ BigDye™ Terminator v3.1 Cycle Sequencing Kit
The BigDye™ Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry
Product Type | Sequencing Buffer |
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For Use With (Equipment) | 310 Genetic Analyzer, 3130xl Genetic Analyzer, 3730xl DNA Analyzer, GeneAmp 9700, Veriti Thermal Cycler, 3130 Genetic Analyzer, 3730 DNA Analyzer, 3500xL Genetic Analyzer, 3500 Genetic Analyzer |
Applied Biosystems™ POP-7™ Polymer for 3730/3730xl DNA Analyzers
POP-7™ Polymer is a separation matrix for the 3730 and 3730xl DNA Analyzers. POP-7™ Polymer is customized for DNA sequencing and fragment analysis.
Accuracy | 98.5% up to 900 bases (for 50 cm capillary), 98.5% up to 700 bases (for 36 cm capillary) |
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Product Type | Polymer |
For Use With (Application) | Standard Sequencing, Fragment Analysis (Denaturing) |
Test Time | 1 hr for 36 cm capillary (std. seq.), 2 hrs for 50 cm capillary (long read seq.) |
Product Line | POP-7™ |
Invitrogen™ Collibri™ Library Quantification Kit
Invitrogen™ Collibri™ Library Quantification Kit includes a ready-to-use master mix optimized for Illumina NGS library quantification and a library dilution buffer
Shipping Condition | Dry Ice |
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Polymerase | Platinum™ II Taq Hot Start DNA Polymerase |
GC-Rich PCR Performance | Low |
Content And Storage | Store at -20°C. |
Format | Kit |
Product Type | Library Quantification Kit |
For Use With (Equipment) | 7500 Fast System, 7900HT Fast System, QuantStudio™, ViiA™ 7 System, StepOne™ System, StepOnePlus™ System |
Sample Type | DNA,RNA |
PCR Method | qPCR |
For Use With (Application) | Illumina NGS Library Quantification |
Test Time | Standard |
Product Line | Collibri™ |
Detection Method | SYBR |
Applied Biosystems™ BigDye™ Direct Cycle Sequencing Kit
The BigDye™ Direct Cycle Sequencing Kit, part of the BigDye™ product family, is a complete kit for your PCR and Sequencing needs.
Shipping Condition | Dry Ice |
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Format | 8-well Strip,96-well Plate |
Product Type | Direct Cycle Sequencing Kit |
Technique | Fluorescent Dye Terminator Sequencing |
For Use With (Application) | Comparative Sequencing (Germline Mutations - 30:70 Heterozygotes), Comparative Sequencing (Germline Mutations - 50:50), GC-Rich Sequencing (>65%), GT-Rich or Difficult Template Sequencing, Standard Sequencing, Gap Closure, Homopolymer A or T, AT-Rich Sequencing (>65%), Comparative Sequencing (Germline Mutations -10:90 Heterozygotes) |
Template Compatibility | BAC DNA, Cosmids, Lambda DNA, PCR Amplicons (10:90 Heterozygotes), Plasmid DNA (≤15Kb), Genomic DNA (Bacterial), Fosmids, PCR Amplicons (50:50 Heterozygotes), PCR Amplicons (30:70 Heterozygotes) |
Product Line | BigDye™ |
Invitrogen™ GeneRacer™ Kit with SuperScript™ III RT and TOPO TA Cloning™ Kit for Sequencing
Ensures amplification of only full-length transcripts via elimination of truncated messages from amplification process
Invitrogen™ GeneArt™ Gibson Assembly EX Master Mix
GeneArt Gibson Assembly EX Master Mix enables DNA assembly via a technique that allows multiple overlapping DNA fragments to be seamlessly linked in a single-tube, two-step optimized reaction.
Shipping Condition | Dry Ice |
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Content And Storage | Includes master mix, positive control, and water and accommodates the use of your own competent cells. |
Workflow Step | DNA Assembly |
Number of Fragments | Up to 15 fragments |
Product Type | Master Mix |
Control Type | Positive Control |
Cloning Method | Seamless cloning |
For Use With (Application) | Seamless Cloning & Genetic Assembly |
Product Line | GENEART™ |