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Cytiva HiTrap™ IEX HP Prepacked Columns
Use for high-resolution, small-scale protein purification. Cytiva Life Sciences™ HiTrap™ IEX HP Prepacked Columns are prepacked, ready-to-use Sepharose™ High Performance strong cation exchange columns.
| Column Type | Column Accesory |
|---|---|
| Material | Polypropylene |
Cytiva HiTrap™ Heparin HP Columns
Purify DNA-binding proteins, coagulation factors, lipoproteins and protein synthesis factors. Cytiva Life Sciences™ HiTrap™ Heparin HP Columns are prepacked with Heparin Sepharose™ High Performance for high-resolution purification.
| Particle Size | 34 μm |
|---|---|
| Ligand Type | 10mg Heparin/mL medium |
| Height (Metric) | 2.5cm |
| Content And Storage | 4° to 30°C |
| pH Range | 5 to 10 |
| Product Type | Heparin HP Column |
| For Use With (Equipment) | Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ design or FPLC system |
| Material | Polypropylene |
| For Use With (Application) | Purification of Antithrombin III, coagulation facto rs and other Plasma Proteins |
| Support Type | 6% Highly Cross-linked Agarose |
| Max. Pressure | 42psi |
| Height (English) | 0.98 in. |
Cytiva HiTrap™ Protein G HP Prepacked Columns
Use for fast, easy separations of IgG from different species and subclasses. Cytiva Life Sciences™ HiTrap™ Protein G HP Prepacked Columns are prepacked with Protein G Sepharose™.
| Content And Storage | 4°C to 30°C |
|---|---|
| Description | HiTrap Protein G HP are prepacked columns for general-purpose preparative purification of monoclonal and polyclonal antibodies from most species including rat. |
| Storage Buffer | 20% Ethanol |
| Product Type | Pre-packed Columns |
| For Use With (Application) | Designed for use with a syringe, peristaltic pump, or chromatography system |
Cytiva HiTrap™ DEAE Sepharose Fast Flow IEX Columns
Weak anion exchanger for small-scale protein purifications. Cytiva Lifescience™ HiTrap™ DEAE Sepharose Fast Flow IEX Columns are convenient and rapid.
| Content And Storage | 4°C to 30°C in 20% Ethanol |
|---|---|
| Description | HiTrap DEAE FF |
| For Use With (Application) | For method scouting, group separations, sample concentration and sample clean-up of charged biomolecules |
Cytiva HiTrap™ TALON™ Crude Prepacked Columns
For purification of histidine-tagged recombinant proteins by immobilized metal affinity chromatography (IMAC). Cytiva Lifescience™ HiTrap™ TALON™ Crude Prepacked Columns offer a cobalt-based medium allowing protein purification under native or denaturing conditions.
| Particle Size | 60 to 160 μm |
|---|---|
| Ligand Type | Talon Superflow Cobalt-IMAC |
| Flow Rate | 1 to 4 mL/min |
| Matrix | 6% Cross-Linked Agarose |
| Storage Buffer | 20% Ethanol |
| pH Range | 3 to 12 |
| Product Type | HiTrap Talon crude prepacked Column |
| Material | Polypropylene |
| Storage Requirements | 4°C to 8°C |
| For Use With (Application) | For PurifiCatIon of Histidine-Tagged Recombinant Proteins by Immobilized Metal Affinity Chromatography, Used Buffers and Easily Regenerated with 0.5 M NaOH |
| Max. Pressure | 3 bar |
Cytiva HiTrap Capto MMC Columns
HiTrap Capto MMC is prepacked with Capto MMC, a multimodal BioProcess ion exchange medium. The multimodal functionality gives a different selectivity compared with traditional ion exchangers and it binds proteins at high or low ionic strength.
| Particle Size | 75μm |
|---|---|
| Buffer | Aqueous Buffer |
| Height (Metric) | 25mm |
| Content And Storage | 4° to 30°C |
| pH Range | 2 to 12 |
| Product Type | HiTrap Capto MMC column |
| Column Type | Affinity Column |
| Material | Polypropylene |
| For Use With (Application) | Bind Proteins at the conductivity of the feed material and to solve specific purification challenges |
| Support Type | Highly Cross-linked Agarose |
| Height (English) | 0.98 in. |
Cytiva HiTrap™ IgM Purification HP Column
Optimized for fast, efficient purification of monoclonal IgM from hybridoma cell culture
Cytiva HiTrap™ Q Sepharose Fast Flow IEX Columns
Use for small-scale protein purifications. Cytiva Life Sciences™ HiTrap™ Q Sepharose Fast Flow IEX Columns are strong anion exchangers for rapid separations either alone or connected in series.
| Content And Storage | 4°C to 30°C in 20% Ethanol |
|---|---|
| Description | HiTrap Q FF |
Cytiva HiTrap™ SP Sepharose Fast Flow IEX Columns
Use for small-scale protein purifications Cytiva Life Sciences™ HiTrap™ SP Sepharose Fast Flow IEX Columns are strong cation exchanger columns for rapid separations either alone or connected in series.
| Content And Storage | 4°C to 30°C, 0.2M Sodium Acetate in 20% Ethanol |
|---|---|
| Description | HiTrap SP FF |
Cytiva HiTrap™ CM Sepharose Fast Flow IEX Columns
Weak cation exchanger for small-scale protein purifications
| Content And Storage | 4°C to 30°C in 20% Ethanol |
|---|---|
| Description | HiTrap CM FF |
| For Use With (Application) | For method scouting, group separations, sample concentration and sample clean-up of charged biomolecules |
Cytiva HiTrap™ Q Sepharose XL IEX Columns
Optimized for fast, convenient small-scale protein capture
| Content And Storage | 4°C to 30°C in 20% Ethanol |
|---|---|
| Description | HiTrap Q XL |
| For Use With (Application) | HiTrap Q XL are strong anion exchangers prepacked with Q Sepharose XL media, optimized for fast, convenient small-scale protein capture using ion exchange (IEX) chromatography. |
Cytiva HiTrap™ Capto™ SP ImpRes IEX Columns
High-throughput medium for intermediate and polishing protein purification
| Content And Storage | 4°C to 30°C, 0.2M Sodium Acetate in 20% Ethanol |
|---|---|
| For Use With (Application) | HiTrap Capto SP ImpRes chromatography column is packed with a strong cation exchange modern resin for high resolution, small-scale protein purification. |
| Diameter (Metric) Inner Column | 7 mm |
Cytiva HiTrap™ NHS-Activated HP Columns
Couple ligands containing primary amines easily. Cytiva Life Sciences™ HiTrap™ NHS-Activated HP Columns are prepacked with NHS activated Sepharose™ High Performance.
| Particle Size | 34 μm |
|---|---|
| Ligand Type | 10μM NHS groups/mL medium |
| Height (Metric) | 2.5cm |
| Content And Storage | 4° to 8°C |
| pH Range | 3 to 12 |
| Product Type | NHS-Activated HP Column |
| For Use With (Equipment) | Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ design or FPLC system |
| Material | Polypropylene |
| For Use With (Application) | Preparative affinity chromato graphy, easy coupling of ligands containing primary Amines |
| Support Type | 6% Highly Cross-linked Agarose |
| Max. Pressure | 42psi |
| Height (English) | 0.98 in. |
Cytiva HiTrap™ Phenyl HP Columns
Prepacked columns for convenient and fast separations. Cytiva Lifescience™ HiTrap™ Phenyl HP Columns are designed for use with a syringe, laboratory pump or chromatography system.
| Particle Size | 24 to 44 μm |
|---|---|
| Ligand Type | Phenyl |
| pH Range | 3 to 13 |
| Product Type | HiTrap Phenyl HP |
| Material | Polypropylene |
| Storage Requirements | 4°C to 30°C, 20% Ethanol |
| For Use With (Application) | For Screening of Selectivity, Binding and Elution Conditions, as Well as Small Scale Purifications |
| Support Type | 6% Cross-linked Agarose |
| Media Type | Phenyl Sepharose HP |
| Max. Pressure | 5 bar [0.5 MPa] (70 psi) |