Effectively screen colonies with X-Gal, an inert chromogenic substrate for beta-galactosidase which hydrolyzes X-Gal, forming an intense blue precipitate.
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Effectively screen colonies with X-Gal, an inert chromogenic substrate for beta-galactosidase which hydrolyzes X-Gal, forming an intense blue precipitate.
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Detect expression of the URA3 gene, which encodes orotine-5'-monophosphate (OMP) dicarboxylase with Thermo Scientific™ 5-Fluoroorotic Acid, used in yeast molecular genetics.
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Sheared Salmon Sperm DNA is a very effective blocking agent when used in Northern prehybridization and hybridization buffers at a concentration of 100 to 200 μg/mL.