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Hot Start PCR Reagents and Kits

In hot start PCR, key reagents are prevented from reacting in the PCR mixture until optimal temperatures are met. This reduces non-specific DNA amplification that occurs naturally at lower temperatures, increasing product yield, specificity, and sensitivity.
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Thermo Scientific™ Phire Hot Start II PCR Master Mixes

Thermo Scientific Phire Hot Start II PCR Master Mix is convenient 2X mix designed to minimize the number of pipetting steps. The master mix contains Phire Hot Start II DNA Polymerase, nucleotides and optimized reaction buffer including MgCl2.

EDGE
Shipping Condition Wet or Dry Ice
Polymerase Phire Hot Start II DNA Polymerase
GC-Rich PCR Performance Medium
Fidelity (vs. Taq) 2X
Reaction Format SuperMix or Master Mix
Product Type Hot Start PCR Master Mix
Reaction Speed Fast
Hot Start Built-In Hot Start
Overhang Blunt
Concentration 2X
Starting Material DNA
For Use With (Application) Cloning,Hot-start PCR
2

DreamTaq™ Hot Start PCR Master Mixes

Thermo Scientific DreamTaq Hot Start PCR Master Mix is a ready-to-use 2X reaction mix that includes DreamTaq Hot Start DNA Polymerase, DreamTaq buffer, magnesium, and dNTPs.

EDGE
Shipping Condition Dry Ice
Polymerase DreamTaq DNA Polymerase
GC-Rich PCR Performance Low
Reaction Format SuperMix or Master Mix
Reaction Speed Standard
Hot Start Built-In Hot Start
Overhang 3'-A
Concentration 2X
For Use With (Application) Hot-start PCR
3

Invitrogen™ Platinum™ II Hot-Start PCR Master Mixes (2X)

Invitrogen Platinum II Hot-Start PCR Master Mix (2X) offers Platinum II Taq Hot-Start DNA Polymerase premixed with Platinum II PCR buffer and dNTPs for convenient PCR setup.

EDGE
GC-Rich PCR Performance High
Fidelity (vs. Taq) 1X
Format Tube
Size (Final Product) 5 kb or less
Hot Start Built-In Hot Start
Concentration 2X
For Use With (Application) Hot-start PCR
4

Invitrogen™ Platinum™ Direct PCR Universal Master Mix
GREENER_CHOICE

Invitrogen Platinum Direct PCR Universal Master Mix is designed to amplify DNA directly from various samples without the need to purify DNA.

EDGE
Shipping Condition Dry Ice
Polymerase Platinum II Taq Hot-Start DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) 1X
Size (Final Product) 8 kb
Reaction Format SuperMix or Master Mix
Product Type Direct PCR Universal Master Mix
Reaction Speed Fast
Hot Start Built-In Hot Start
Overhang 3'-A
Green Features Less hazardous, less waste
Concentration 2X
For Use With (Application) Hot-start PCR,Real Time PCR (qPCR)
5

Invitrogen™ Platinum™ Hot Start PCR Master Mixes (2X)

Invitrogen Platinum Hot Start PCR Master Mix offers Platinum Taq DNA polymerase in an optimized PCR buffer with magnesium and dNTPs for convenient PCR setup.

Shipping Condition Dry Ice
GC-Rich PCR Performance Medium
Fidelity (vs. Taq) 1X
Format Tube
Size (Final Product) 5 kb or less
Reaction Format SuperMix or Master Mix
Product Type Hot Start PCR Master Mix
Reaction Speed Standard
Hot Start Built-In Hot Start
Overhang 3'-A
Concentration 2X
Starting Material DNA
For Use With (Application) Hot-start PCR
6

DreamTaq™ Hot Start PCR Master Mix

Thermo Scientific DreamTaq Hot Start DNA Polymerase is the product of choice when you need consistently robust amplification with minimal optimization steps

7

Invitrogen™ Platinum™ SuperFi™ Green PCR Master Mix

Invitrogen™ Platinum™ SuperFi™ DNA Polymerase is a proofreading DNA polymerase which combines superior fidelity with trusted PlatinumTM hot-start technology for the highest success in PCR.

Shipping Condition Approved for shipment on Wet or Dry Ice
Polymerase Platinum SuperFi DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) 100 X
Reaction Format SuperMix or Master Mix
Reaction Speed Fast
Hot Start Built-In Hot Start
Overhang Blunt
Product Line Platinum, SuperFi