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TRA-1-81 (Podocalyxin) Monoclonal Antibody (TRA-1-81), eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
109.00 EUR - 280.00 EUR
Specifications
Clone | TRA-1-81 |
---|---|
Concentration | 0.5 mg/mL |
Classification | Monoclonal |
Conjugate | Unconjugated |
Form | Liquid |
Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
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Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
15298107
|
Affymetrix eBioscience
14-8883-82 |
100 μg |
280.00 EUR
100µg |
Estimated Shipment: 29-05-2024 Log in to see stock. |
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15288107
|
Affymetrix eBioscience
14-8883-80 |
25 μg |
This item is currently unavailable or has been discontinued. View the product page for possible alternatives. |
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Description
Description: The TRA-1-81 antibody recognizes a protein expressed on undifferentiated human embryonic stem cells (ES), embryonic carcinoma cells (EC), and embryonic germ cells (EG). Like other stem cell specific markers, the epitope recognized by the TRA-1-81 antibody is lost upon cell differentiation. The TRA-1-81 epitope is resistant to neuraminidase digestion, unlike the epitope recognized by the related TRA-1-60 antibody. The TRA-1-81 antibody is known to specifically recognize a carbohydrate epitope on a keratan sulfated glycoprotein recently identified as podocalyxin, a member of the CD34-related family of sialomucins. Podocalyxin is a transmembrane glycoprotein originally identified on epithelial glomerular cells known as podocytes, and the protein has also been implicated in the development of aggressiveness in a variety of cancers, including breast and prostate cancer. Applications Reported: This TRA-1-81 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting, and immunofluorescence. Applications Tested: This TRA-1-81 antibody has been tested by flow cytometric analysis of the human embryonic carcinoma (EC) line 2102Ep. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
TRA-1-81 is a cell surface antigen expressed along with SSEA-3, SSEA-4 and TRA-1-60 in human embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). These surface markers are down-regulated during the differentiation process. In contrast, SSEA-1 is absent in undifferentiated human stem cells but is present on the cell surface after retinoic acid mediated differentiation.Specifications
TRA-1-81 | |
Monoclonal | |
Liquid | |
RUO | |
IgM | |
Affinity chromatography | |
Antibody |
0.5 mg/mL | |
Unconjugated | |
Mouse | |
Human | |
Primary | |
4° C |
For Research Use Only.