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TRA-1-81 (Podocalyxin), clone: TRA-1-81, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-8883-80

Additional Details : Weight : 0.01000kg

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Product Code. 15288107

  • 99.25 EUR / 25µg


Estimated Shipment date
from Supplier 04-06-2019
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Description

Description

This antibody has also been tested by immunoblotting of 2102Ep cell extracts, yielding a band of approximately 200-250 kDa (reducing and non-reducing conditions). This antibody has been tested by immunofluorescent staining of formaldehyde fixed and permeabilized cells and can be used at less than or equal to 5µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered. TRA-1-81 is a cell surface antigen expressed along with SSEA-3, SSEA-4 and TRA-1-60 in human embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). These surface markers are down-regulated during the differentiation process. In contrast, SSEA-1 is absent in undifferentiated human stem cells but is present on the cell surface after retinoic acid mediated differentiation.
Specifications

Specifications

TRA-1-81 (podocalyxin)
Monoclonal
0.5 mg/mL
PBS with 0.09% sodium azide; pH 7.2
Keratan sulfate antigen TRA1-81; TRA180
IgM
25 μg
4° C
Primary
Human
Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
TRA-1-81
Unconjugated
Liquid
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
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