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TRA-1-81 (Podocalyxin), clone: TRA-1-81, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-8883-80

Additional Details : Weight : 0.01000kg

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Product Code. 15288107

  • 97.25 EUR / 25µg


Estimated Shipment date
from Supplier 24-10-2018
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Description

Description

Description: The TRA-1-81 antibody recognizes a protein expressed on undifferentiated human embryonic stem cells (ES), embryonic carcinoma cells (EC), and embryonic germ cells (EG). Like other stem cell specific markers, the epitope recognized by the TRA-1-81 antibody is lost upon cell differentiation. The TRA-1-81 epitope is resistant to neuraminidase digestion, unlike the epitope recognized by the related TRA-1-60 antibody. The TRA-1-81 antibody is known to specifically recognize a carbohydrate epitope on a keratan sulfated glycoprotein recently identified as podocalyxin, a member of the CD34-related family of sialomucins. Podocalyxin is a transmembrane glycoprotein originally identified on epithelial glomerular cells known as podocytes, and the protein has also been implicated in the development of aggressiveness in a variety of cancers, including breast and prostate cancer.Applications Reported: This TRA-1-81 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting, and immunofluorescence.Applications Tested: This TRA-1-81 antibody has been tested by flow cytometric analysis of the human embryonic carcinoma (EC) line 2102Ep. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. This antibody has also been tested by immunoblotting of 2102Ep cell extracts, yielding a band of approximately 200-250kDa (reducing and non-reducing conditions). This antibody has been tested by immunofluorescent staining of formaldehyde fixed and permeabilized cells and can be used at less than or equal to 5μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

TRA-1-81 (podocalyxin)
Monoclonal
0.5mg/mL
PBS with 0.09% sodium azide; pH 7.2
Keratan sulfate antigen TRA1-81, TRA180
IgM
25μg
Store at 2-8°C.
Primary
Human
Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
TRA-1-81
Unconjugated
Liquid
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
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