TRA-1-81 (Podocalyxin), clone: TRA-1-81, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 14-8883-80
Additional Details : Weight : 0.01000kg
DescriptionThis antibody has also been tested by immunoblotting of 2102Ep cell extracts, yielding a band of approximately 200-250 kDa (reducing and non-reducing conditions). This antibody has been tested by immunofluorescent staining of formaldehyde fixed and permeabilized cells and can be used at less than or equal to 5µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered. TRA-1-81 is a cell surface antigen expressed along with SSEA-3, SSEA-4 and TRA-1-60 in human embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). These surface markers are down-regulated during the differentiation process. In contrast, SSEA-1 is absent in undifferentiated human stem cells but is present on the cell surface after retinoic acid mediated differentiation.
|PBS with 0.09% sodium azide; pH 7.2|
|Keratan sulfate antigen TRA1-81; TRA180|
|Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot|
For Research Use Only.