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Thermo Scientific™ Sino Biological™ BACE1 Recombinant Mouse Protein, His Tag
This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Thr 457) of the mouse BACE1 precursor (NM_011792.5) fused to the polyhistidine tag at the C-terminus
Brand: Thermo Scientific™ 50002M08H250
Additional Details : Weight : 0.01000kg
Description
Beta-site APP-cleaving enzyme 1 (BACE1), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.- Activity: Measured by its ability to cleave a fluorescent peptide substrate Mca-Ser-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Arg-Lys(Dpn)-Arg-Arg-NH2, cleavage of this substrate can be measured using excitation and emission wavelengths of 320 and 405nM, respectively; the specific activity is >2pmole/min./μg
- Formulation: Lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
- Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible
This protein is manufactured by Sino Biological Inc.
Specifications
| Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories | |
| 49.8kDa | |
| 5 x 50μg | |
| Proteases | |
| Bace1, C76936 | |
| BACE1 | |
| Other Proteins | |
| His Tag |
| Lyophilized | |
| ≥90% by SDS-PAGE | |
| Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed. | |
| <1 EU/μg | |
| beta-site APP cleaving enzyme 1 | |
| Assay Standard | |
| Mammalian |
For Research Use Only. Not for use in diagnostic procedures.