ROR gamma (t), PerCP-eFluor™ 710, clone: B2D, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 46-6981-80
Additional Details : Weight : 0.25000kg
DescriptionDescription: The B2D monoclonal antibody reacts with the mouse ROR gamma (t) protein. RORgamma is a member of the retinoic acid-related orphan receptor (ROR) family, which also includes ROR alpha and ROR beta. ROR family proteins are ligand-dependent transcription factors that play roles in multiple physiological processes. ROR gamma is expressed in several tissues including liver, lung, muscle, heart and kidney. Furthermore, it was discovered that alternative transcription results in the expression of an isoform, ROR gamma t, which is expressed exclusively in cells of the lymphoid compartment, namely CD4+CD8+ double-positive thymoyctes, Th17 cells of the periphery and lymphoid tissue inducer (Lti) cells of lymphoid organs.The ROR gamma t isoform differs from RORgamma by three unique amino acids at the amino terminus. Therefore, the B2D antibody will react with both the ROR gamma and ROR gamma t isoforms.Applications Reported: This B2D antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This B2D antibody has been tested by intracellular staining and flow cytometric analysis of mouse thymocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative. Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
|ROR gamma (t)|
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|MGC129539, NR1F3, RORG, RZR-GAMMA, RZRG, TOR|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.