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Invitrogen™ PolarScreen™ PPARγ-Competitor Assay Kit, Green

PolarScreen™ PPARγ-Competitor Assay Kit, Green

Brand:  Invitrogen™ PV6136

Additional Details : Weight : 0.09000kg

Product Code. 11632159

  • 1582.00 EUR / 16000µg
Estimated Shipment: 18-04-2024
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Description

Description

The PPARγ (Peroxisome Proliferator-Activated Receptor-gamma) Competitor Assay, Green uses the human-derived recombinant PPARγ ligand-binding domain (PPARγ-LBD) tagged with an N-terminal GST-tag and a novel, tight-binding, selective fluorescent PPARγ ligand (Fluormone™ PPARγ Green) in a homogenous, mix-and-read assay format. PPARγ-LBD/Fluormone™ PPARγ Green complex produces high polarization. This complex is then added to individual test compounds in microwell plates. Competitors displace the fluorescent Fluormone™ PPARγ Green Ligand from the PPARγ-LBD/Fluormone™ PPARγ Green complex, causing the fluorescent ligand to tumble rapidly during its fluorescence lifetime, resulting in a low polarization value. Noncompetitors will not displace the fluorescent ligand from the complex, so the polarization value remains high. The shift in polarization value in the presence of the test compounds is used to determine relative affinity of test compounds for the PPARγ-LBD.
Specifications

Specifications

Green
Microplate Reader
PV3356 — Fluormone™ PPARγ Green (-20°C)
PV4546 — PPARγ-LBD (-80°C)
PV3358 — PPARγ Green Screening Buffer (room temperature)
P2325 — 1M DTT (-80°C or -20°C)
485/535
End Point
PPARG, PPAR gamma, NR1C3
Competitive Binding Assay, Fluorescence Polarization
Assay Kit
Fluorescent
384-well Plate
Biochemical competitive binding
5468
Dry Ice
PolarScreen™
PPAR
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For Research Use Only. Not for use in diagnostic procedures.