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PIR-A/B, APC, clone: 10-1-PIR, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 17-3101-80

Additional Details : Weight : 0.25000kg

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Product Code. 15390710

  • 88.25 EUR / 25µg


Estimated Shipment date
from Supplier 26-02-2018
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Disclaimers:

For Research Use Only.


Description

Description

Description: This 10-1-PIR monoclonal antibody reacts with mouse paired Ig-like receptors of activating (PIR)-A and -B. These cell surface glycoproteins, which contain six extracellular Ig-like domains with distinct transmembrane and cytoplasmic regions, are expressed together on B lymphocytes, dendritic cells, macrophages, granulocytes, platelets, and mast cells. However, expression of PIR-A/B has not been observed on T cells, NK cells, and erythrocytes. The PIR-A receptor interacts with signaling molecules containing immunoreceptor tyrosine-based activation motifs (ITAMs) which lead to its activating function. In contrast, the PIR-B receptor associates with proteins possessing immunoreceptor tyrosine-based inhibitory motifs (ITIMs); therefore, this receptor has been shown to have an inhibitory function. PIRs bind MHC class I to modulate cell signaling and homeostasis of the immune system. Moreover, PIR-B knockout mice have been shown to exhibit susceptibility to Salmonella infection.Crossblocking studies indicate that 10-1-PIR recognizes a different epitope from 6C1 (cat. no. 46-5978).Applications Reported: This 10-1-PIR antibody has been reported for use in flow cytometric analysis.Applications Tested: This 10-1-PIR antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

PIR-A/B
10-1-PIR
APC
Conjugated
Rat
25μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
Flow Cytometry
0.2mg/mL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
PirA, PirB, PIR A, PIR-A, PIR B, PIR-B, PIR AB, PIR-AB, PIRAB
IgG2b
RUO
Primary
Murine
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