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OPTIZYME™ TaqI, Fisher BioReagents™

Brand:  Fisher Bioreagents BP8007-1

Additional Details : Weight : 0.01000kg

Product Code. 11964842

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Description

Description

5'...T^C G A...3'
3'...A G C^T...5'

Supplied with: 10X OPTIZYME TaqI Buffer
Conditions for 100% Activity:

  • 1X OPTIZYME Buffer TaqI: 10mM Tris-HCl (pH 8.0 at 37°C), 5mM MgCl2, 100mM NaCl and 0.1mg/ml BSA
  • Incubate at 65°C.
  • To ensure higher efficiency digestion, perform the cleavage reaction under paraffin oil.
Enzyme Activity in OPTIZYME buffers:
  • Buffer 1: 0 - 20%
  • Buffer 2: 20 - 50%
  • Buffer 3: 20 - 50%
  • Buffer 4: 20 - 50%
  • Buffer 5: 20 - 50%
Storage Buffer:
  • 10mM Tris-HCl (pH 7.5 at 25°C), 300mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/ml BSA and 50% (v/v) glycerol
l
  • Ligation and Re-cleavage:
  • More than 95% of DNA fragments can be ligated and re-cut after a 50-fold over-digestion with TaqI.
  • Notes:
    • TaqI is blocked by overlapping dam methylation.
    • To avoid dam methylation, use a dam-, dcm- strain.
    • Incubation at 37°C results in 10% activity.
    Compatible Ends: Bsp119I, Bsu15I, Hin1I, Hin6I, HpaII, MaeII, MspI, NarI, Psp1406I, SsiI, XmiI
    Methylation Effects:
    Dam: May overlap - blocked
    • 5'...TCGm6A TC...3'
  • 3'...AGC Tm6AG...5'
  • (Cleavage is blocked)
  • Dcm: Never overlaps - no effect
  • CpG: Completely overlaps - no effect
    • TCGA
  • (No effect on cleavage)
  • EcoKI: Never overlaps - no effect
  • EcoBI: Never overlaps - no effect
  • Specifications

    Specifications

    10 U/μL
    65°C
    >95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with TaqI
    3000 U
    Taql
    10X OPTIZYME™ TaqI Buffer
    7.4
    10mM Tris HCl (pH 7.5 at 25°C), 300mM KCl, 0.1mM EDTA, 1mM DTT, 0.5mg/mL BSA, 50% Glycerol
    T.CGA
    Liquid
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