Invitrogen™ Dynabeads™ His-Tag Isolation and Pulldown
Designed for his-tagged protein isolation
Brand: Invitrogen™ 10104D
Additional Details : Weight : 0.10500kg
Requires : Buffers
High selectivity for poly-histidine tags results in extremely low background levels. Bead surface chemistry is cobalt-based and offers exceptionally fast binding kinetics and a high yield per microliter of beads. The entire protocol (from cleared lysate to purified protein) takes only 20 minutes. Protocol includes instructions for making the required buffers and methods for optimizing buffer composition. Dynabeads His-Tag Isolation and Pulldown replaces Dynabeads TALON™. Dynabeads His-Tag Isolation and Pulldown has a optimized binding chemistry that offers significant advantages over Dynabeads TALON which were coated in the TALON chemistry. This optimization results in a cleaner prep with higher yield of his-tag protein and extremely low background levels. Dynabeads His-Tag Isolation and Pulldown is designed for isolating His-tagged proteins from cleared lysates. Isolated his-tagged proteins can either be eluted from the beads or kept on the beads for use in bead-based downstream assays. Bead bound his-tagged proteins can be used in various downstream assays such as bait-prey pulldowns, etc.
Technology used for discontinued Dynabeads TALON was licensed from Clontech. TALON is a registered trademark of Clontech Laboratories Inc., USA.
His-Tagged Protein Purification, Protein and Peptide Purification, Protein Complex Pull-Down, Protein Sample Preparation and Protein Purification, Proteins, Expression, Isolation and Analysis
|Dynabeads™ His-Tag Isolation and Pulldown beads are supplied as a suspension supplied in 20% ethanol. Store at: 2-8°C|
|His-tagged protein pull-down|
|High-throughput Compatible, Not High-throughput Compatible (Manual), Automated Protocols|
|Cell Cultures, Tissue Digests, Intact Proteins, Cell Lysates, Cell Extracts|
|For Research Use Only|
For Research Use Only. Not for use in diagnostic procedures.