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Invitrogen™ Mouse MCP-1 ELISA Kits
Measure levels of mouse monocyte chemoattractant protein-1 (MCP1) in mouse serum, plasma and cell culture supernatant samples using 96-well plates.
Brand: Invitrogen™ EMMCP1
Additional Details : Weight : 0.20000kg
Description
Assay MCP-1 (mouse monocyte chemoattractant protein-1) levels in mouse serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples with our Thermo Scientific™ Mouse MCP-1 ELISA Kits.
These mouse MCP-1 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of monocyte chemoattractant protein-1 (MCP-1) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. The economically-priced reagent kit provides validated reagents only, requiring users to coat their own plates. All components undergo quality control testing to minimize lot-to-lot variability.Target: Natural and recombinant mouse MCP-1 (monocyte chemoattractant protein-1)
Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Measurement: Absorbance at 450nm minus absorbance at 550nm
Assay Range: 16 to 1000pg/mL
Sensitivity: < 4pg/mL
Precision: Intra-assay CV < 10%; inter-assay CV < 10%
Sample Types: Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
Total Assay Time: 3 hours
Sample Size: 50μL
Specificity: No significant cross-reaction with mouse G-CSF, GM-CSF, IFNγ, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-18, Eotaxin, MIP-1α, RANTES or TNFα, or human or rat MCP-1
Includes:Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Reagent Kit (previously called a “Screening Set”) contains matched antibodies, standard and detection reagents but no coated plates.
Specifications
| 16 to 1000pg/mL | |
| Mouse MCP-1 ELISA Kit | |
| Cell Culture Supernatant, Plasma (EDTA, Citrate, Heparin), Serum | |
| 96 microplate wells | |
| Absorbance at 450nm minus absorbance at 550nm | |
| 1 Plate Kit | |
| Natural and recombinant mouse MCP-1 (monocyte chemoattractant protein-1) |
| <4pg/mL | |
| Colorimetric ELISA (TMB substrate) with Plates | |
| No significant cross-reaction with mouse G-CSF, GM-CSF, IFNg, IL-1a, IL-1ß, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-18, Eotaxin, MIP-1a, RANTES or TNFa, or human or rat MCP-1 | |
| TMB Substrate Solution, 12mL, Streptavidin-HRP Concentrate, 75µL, Stop Solution, 13mL, 30X Wash Buffer, 50mL, Adhesive Plate Covers, 6 ea, Anti-Mouse MCP-1 Precoated 96-well Strip Plate, 1 ea, Biotinylated Antibody Reagent, 12mL, HRP Dilution Buffer, 13mL, Lyophilized Recombinant Mouse MCP-1 Standard, 2 vials, Sample Diluent, 12mL | |
| Intra-assay CV <10%; inter-assay CV <10% | |
| 50 μL | |
| 3 hr. |