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Invitrogen™ LanthaScreen™ TR-FRET RAR beta Coactivator Assay Kit, goat

LanthaScreen™ TR-FRET RAR beta Coactivator Assay Kit, goat

Brand:  Invitrogen™ PV4688

Additional Details : Weight : 0.09000kg

Product Code. 10286503

  • 1740.00 EUR / Pack of 800
Estimated Shipment: 18-04-2024
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Description

Description

This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15137:

The LanthaScreen™ TR-FRET RAR (retinoic acid receptor) beta Coactivator Assay Kit provides a sensitive and robust method for highthroughput screening of potential RAR beta ligands as agonists of ligand-dependent coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a human recombinant RAR beta ligand-binding domain (RAR beta-LBD) that is tagged with glutathione-S-transferase (GST) in a homogeneous, mix-and-read assay format.

When running the LanthaScreen™ TR-FRET RAR beta coactivator assay in agonist mode to identify agonist compounds, RAR beta-LBD is added to ligand test compounds, followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-labeled anti-GST antibody. After an incubation period at room temperature, the 520 nm/495 nm TR-FRET ratio is calculated and can be used to determine the EC50 from a dose response curve of the compound. Based on the biology of the RAR beta-coactivator peptide interaction, this ligand EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and recruit coactivator peptide (Figure 1).

Contents and Storage:
The LanthaScreen™ TR-FRET RAR beta Coactivator Assay Kit contains RAR beta-LBD (GST) protein, fluorescently labeled SRC2-2 coactivator peptide, Tb-labeled anti-GST antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C).
Specifications

Specifications

FITC (Fluorescein), Tb (Terbium)
Fluorescent
RAR beta-LBD (GST) protein, fluorescently labeled SRC2-2 coactivator peptide, Tb-labeled anti-GST antibody, and buffers
For research use only (RUO)
-68°C to -85°C
LanthaScreen™ TR-FRET RAR Beta Coactivator Assay Kit
LanthaScreen™ TR-FRET RAR Beta Coactivator Assay Kit, 800 x 20μL Assays, 384-Well Plate Format, FITC (Fluorescein), Tb (Terbium) Dye Type, Fluorescent Detection Method, TR-FRET Technique, For Pharma & Biopharma, Target & Lead Identification
Microplate reader
800 x 20 μL Assays
384-well Plate
Pharma and biopharma, drug discovery and development, target and lead identification and validation, nuclear receptor biology, biochemical nuclear receptor assays, nuclear receptor coregulator interaction
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For Research Use Only. Not for use in diagnostic procedures.