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Invitrogen™ LanthaScreen™ TR-FRET LXR beta Coactivator Assay Kit, rabbit
LanthaScreen™ TR-FRET LXR beta Coactivator Assay Kit (Rabbit)
Brand: Invitrogen™ A15129
Additional Details : Weight : 0.09000kg
Description
This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4658:The LanthaScreen™ TR-FRET LXR (liver X receptor) beta Coactivator Assay provides a sensitive and robust method for high-throughput screening of potential LXR beta ligands as agonists of coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and an LXR beta ligand-binding domain (LBD) that is tagged with glutathione-S-transferase (GST), in a homogeneous mix-and-read assay format.
How it works:
In the LanthaScreen™ TR-FRET LXR beta Coactivator Assay, LXR beta-LBD is added to ligand test compounds followed by the addition of a mixture of the fluorescein-coactivator peptide and Tb-labeled anti-GST antibody. After an incubation period at room temperature, the 520 nm/495 nm TR-FRET ratio is calculated and can be used to determine the EC50 from a dose response curve of the compound. Based on the biology of the LXR beta-coactivator peptide interaction, this ligand EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and recruit coactivator peptide (Figure 1).
Contents and Storage:
The LanthaScreen™ TR-FRET LXR beta Coactivator Assay Kit contains LXR beta-LBD (GST) protein, fluorescently labeled D22 coactivator peptide, Tb-labeled anti-GST antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C).
Specifications
Green | |
Fluorescent | |
384-well Plate | |
Biochemical Assay | |
TR-FRET (Activity) | |
Nuclear Receptor Assay, TR-FRET | |
TR-FRET LXR Beta Coactivator Assay Kit |
Tb (Terbium) | |
Microplate Reader | |
Tb-anti-GST Antibody (Rabbit): Store at -20 °C LXR beta-LBD, GST: Store at -80 °C Fluoroscein Coactivator Peptide: Store at -20 °C TR-FRET Coregulatore Buffer i: Store at 4 °C 1M DTT: Store at -20 °C or -80 °C |
|
Nuclear Receptors | |
LanthaScreen™ | |
LXR Beta |
For Research Use Only. Not for use in diagnostic procedures.