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Invitrogen™ LanthaScreen™ TR-FRET LXR beta Coactivator Assay Kit, rabbit

LanthaScreen™ TR-FRET LXR beta Coactivator Assay Kit (Rabbit)

Brand:  Invitrogen™ A15129

Additional Details : Weight : 0.09000kg

Product Code. 15323796

  • 1994.00 EUR / 1 set
Estimated Shipment: 11-04-2024
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Description

Description

This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4658:

The LanthaScreen™ TR-FRET LXR (liver X receptor) beta Coactivator Assay provides a sensitive and robust method for high-throughput screening of potential LXR beta ligands as agonists of coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and an LXR beta ligand-binding domain (LBD) that is tagged with glutathione-S-transferase (GST), in a homogeneous mix-and-read assay format.

How it works:
In the LanthaScreen™ TR-FRET LXR beta Coactivator Assay, LXR beta-LBD is added to ligand test compounds followed by the addition of a mixture of the fluorescein-coactivator peptide and Tb-labeled anti-GST antibody. After an incubation period at room temperature, the 520 nm/495 nm TR-FRET ratio is calculated and can be used to determine the EC50 from a dose response curve of the compound. Based on the biology of the LXR beta-coactivator peptide interaction, this ligand EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and recruit coactivator peptide (Figure 1).

Contents and Storage:
The LanthaScreen™ TR-FRET LXR beta Coactivator Assay Kit contains LXR beta-LBD (GST) protein, fluorescently labeled D22 coactivator peptide, Tb-labeled anti-GST antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C).
Specifications

Specifications

Green
Fluorescent
384-well Plate
Biochemical Assay
TR-FRET (Activity)
Nuclear Receptor Assay, TR-FRET
TR-FRET LXR Beta Coactivator Assay Kit
Tb (Terbium)
Microplate Reader
Tb-anti-GST Antibody (Rabbit): Store at -20 °C
LXR beta-LBD, GST: Store at -80 °C
Fluoroscein Coactivator Peptide: Store at -20 °C
TR-FRET Coregulatore Buffer i: Store at 4 °C
1M DTT: Store at -20 °C or -80 °C
Nuclear Receptors
LanthaScreen™
LXR Beta
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For Research Use Only. Not for use in diagnostic procedures.