missing translation for 'onlineSavingsMsg'
Learn More
Learn More
Invitrogen™ LanthaScreen™ TR-FRET FXR Coactivator Assay Kit, goat
LanthaScreen™ TR-FRET FXR Coactivator Assay Kit, goat
Brand: Invitrogen™ PV4833
Additional Details : Weight : 0.50000kg
Description
This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15140:The LanthaScreen™ TR-FRET FXR (farnesoid X receptor) Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential FXR ligands as agonists of coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein- labeled coactivator peptide, and an FXR ligand-binding domain (FXR-LBD) that is tagged with glutathione-S-transferase (GST), in a homogeneous, mix-and-read assay format.
To assay:
When running the LanthaScreen™ TR-FRET FXR coactivator assay in agonist mode, FXR-LBD is added to ligand test compounds followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-labeled anti-GST antibody. After an incubation period at room temperature, the 520 nm/495 nm TR-FRET ratio is calculated and used to determine the EC50 from a dose response curve of the compound. Based on the biology of the FXR-coactivator peptide interaction, the ligand EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and recruit coactivator peptide (Figure 1).
Specifications
Tb (Terbium) | |
Microplate Reader | |
The LanthaScreen™ TR-FRET FXR Coactivator Assay Kit contains FXR-LBD (GST) protein, fluorescently labeled SRC2-2 coactivator peptide, Tb-labeled anti-GST antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C). |
|
9971 | |
Dry Ice | |
LanthaScreen™ | |
FXR |
Fluorescent | |
384-well Plate | |
Biochemical coregulator interaction | |
End Point | |
FXR, NR1H4 | |
Co-Factor Interaction Assay, TR-FRET | |
TR-FRET FXR Coactivator Assay Kit |
For Research Use Only. Not for use in diagnostic procedures.