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Invitrogen™ LanthaScreen™ TR-FRET FXR Coactivator Assay Kit, goat

LanthaScreen™ TR-FRET FXR Coactivator Assay Kit, goat

Brand:  Invitrogen™ PV4833

Additional Details : Weight : 0.50000kg

Product Code. 10083053

  • 1760.00 EUR / Each
Estimated Shipment: 17-04-2024
to see stock.
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Description

Description

This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15140:

The LanthaScreen™ TR-FRET FXR (farnesoid X receptor) Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential FXR ligands as agonists of coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein- labeled coactivator peptide, and an FXR ligand-binding domain (FXR-LBD) that is tagged with glutathione-S-transferase (GST), in a homogeneous, mix-and-read assay format.

To assay:
When running the LanthaScreen™ TR-FRET FXR coactivator assay in agonist mode, FXR-LBD is added to ligand test compounds followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-labeled anti-GST antibody. After an incubation period at room temperature, the 520 nm/495 nm TR-FRET ratio is calculated and used to determine the EC50 from a dose response curve of the compound. Based on the biology of the FXR-coactivator peptide interaction, the ligand EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and recruit coactivator peptide (Figure 1).
Specifications

Specifications

Tb (Terbium)
Microplate Reader
The LanthaScreen™ TR-FRET FXR Coactivator Assay Kit contains FXR-LBD (GST) protein, fluorescently labeled SRC2-2 coactivator peptide, Tb-labeled anti-GST antibody, and buffers.

Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C).
9971
Dry Ice
LanthaScreen™
FXR
Fluorescent
384-well Plate
Biochemical coregulator interaction
End Point
FXR, NR1H4
Co-Factor Interaction Assay, TR-FRET
TR-FRET FXR Coactivator Assay Kit
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For Research Use Only. Not for use in diagnostic procedures.