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Invitrogen™ LanthaScreen™ TR-FRET ERR gamma Coactivator Assay Kit, goat
LanthaScreen™ TR-FRET ERR gamma Coactivator Assay Kit, goat
Brand: Invitrogen™ PV4408
Additional Details : Weight : 0.00900kg
Description
This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15121:The LanthaScreen™ TR-FRET Estrogen Related Receptor (ERR) gamma Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential ERR gamma ligands as antagonists/inverse agonists of coactivator displacement. The homogeneous mix-and-read assay format results in a ligand EC50 composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and displace coactivator peptide (Figure 1).
How it works
The LanthaScreen™ TR-FRET ERR gamma Coactivator Assay Kit includes a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a human ERR gamma ligand-binding domain (ERR gamma-LBD) that is tagged with glutathione-S-transferase (GST). To assay, ERR gamma-LBD is added to test compounds, followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-anti- GST antibody. After room temperature incubation, the TR-FRET 520:495 emission ratio is calculated and used to determine the EC50 from a dose response curve of the compound.
Contents and Storage:
The LanthaScreen™ TR-FRET ERR gamma Coactivator Assay Kit contains ERR gamma-LBD (GST) protein, fluorescently labeled PGC1a coactivator peptide, Tb-anti-GST antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C).
Specifications
LanthaScreen™ TR-FRET ERR Gamma Coactivator Assay Kit, 800 x 20μL Assays, 384-Well Plate Format, Tb (Terbium) Dye Type, Fluorescent Detection Method, TR-FRET Technique, For Pharma & Biopharma, Target & Lead Identification & Validation | |
Microplate reader | |
800 x 20 μL Assays | |
384-well Plate | |
Pharma and biopharma, drug discovery and development, target and lead identification and validation, nuclear receptor biology, biochemical nuclear receptor assays, nuclear receptor coregulator interaction |
Fluorescent | |
ERR gamma-LBD (GST) protein, fluorescently labeled PGC1a coactivator peptide, Tb-anti-GST antibody, and buffers | |
For research use only (RUO) | |
-68°C to -85°C | |
LanthaScreen™ TR-FRET ERR Gamma Coactivator Assay Kit |
For Research Use Only. Not for use in diagnostic procedures.