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Invitrogen™ Chargeswitch™ Pro Filter Plasmid Mini Kit
Brand: Invitrogen™ CS31103
Additional Details : Weight : 0.58000kg
Includes: The ChargeSwitch™-Pro Plasmid Miniprep Kit contains Miniprep Columns with Collection Tubes, Recovery Tubes, Resuspension Buffer, RNase A, Lysis Buffer, Precipitation Buffer, Wash Buffers 1 and 2, and Elution Buffer.
Description
The Chargeswitch™ -Pro Filter Plasmid Mini Kit includes 10 plasmid minipreps and provides high yield and high purity plasmid with the fastest and the simplest protocol.The ChargeSwitch™-Pro Plasmid Filter Mini, Midi, Maxi Kits generates high-quality plasmid DNA without the use of harsh or potentially interfering reagents, for better success in downstream applications. The system is based upon a novel dual⁄nested column design which facilitate the fastest and simplest protocol for high quality plasmid. The system is composed of two columns. The inner flat bottomed column, also referred to as “lysate clarification” column, contains filter materials suitable for rapid clarification of bacterial lysate. The outer luer bottomed column, also referred to as “binding” column, contains ChargeSwitch™ (CST) derivatized membrane that immediately binds plasmid DNA from the clarified lysate. This nested-column design enables simultaneous clarification and DNA binding in a single short centrifugation (or vacuum) step. The combined advantages of novel, optimized plastics and a unique chemistry are an extreme competitive advantage of speed and convenience.
To accomplish this, the ChargeSwitch™-Pro Filter Kit provides:
• 100% ethanol-free, guanidinium-free, organics-free, aqueous protocol for a safer, more streamlined method
• Cleaner purified product for improved performance in downstream applications
• A novel Dual⁄nested column design for simultaneous lysate clearing and binding step which offers the fastest and the simplest protocol
• Endotoxin units less than 10 EU ⁄ ug DNA for
• Vacuum Elution can be done with the EveryPrep™ Universal Vacuum Manifold (SKU # K211101)
How it works
The ChargeSwitch™ technology replaces the use of ethanol, chaotropic salts, or organic solvents for nucleic acid purification with a simple ion-exchange mechanism. At low pH values (less than 6.5) the ChargeSwitch™ surface ligand is positively charged and binds plasmid DNA. Raising the pH (greater than 8.5) neutralizes the charge and elutes the bound nucleic acid. The ChargeSwitch™ protocol retains the ease and familiarity of the common silica spin column protocol with a binding, washing, and elution step, making adapting to the ChargeSwitch™ method effortless.
Order Info
Shipping Condition: Room Temperature
Specifications
| Spin Column | |
| Spin Column | |
| Bacterial Culture | |
| 50 to 150 µl | |
| Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription | |
| Miniprep Columns with Collection Tubes, Recovery Tubes, Resuspension Buffer, RNase A, Lysis Buffer, Precipitation Buffer, Wash Buffers 1 and 2, and Elution Buffer | |
| < 100 µg (Small-Scale) Plasmid DNA | |
| Pro Filter Plasmid Mini Kit | |
| Transfection Grade | |
| 100 preps | |
| Plasmid DNA | |
| 15 min |
| The ChargeSwitch™-Pro Plasmid Miniprep Kit contains Miniprep Columns with Collection Tubes, Recovery Tubes, Resuspension Buffer, RNase A, Lysis Buffer, Precipitation Buffer, Wash Buffers 1 and 2, and Elution Buffer. Store at room temperature. Guaranteed stable for 6 months when properly stored. | |
| ChargeSwitch™ Chemistry | |
| Mini | |
| Plasmid DNA | |
| Not High-throughput Compatible (Manual) | |
| 100 Preps | |
| ChargeSwitch™ | |
| 100 Preps | |
| Room Temperature | |
| 5 ml | |
| ChargeSwitch™ | |
| 30 µg |