Integrin alpha 4 beta 7 (LPAM-1), clone: DATK32 (DATK-32), eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 14-5887-85
Additional Details : Weight : 0.01000kg
DescriptionDescription: The DATK32 monoclonal antibody binds to a combinatorial epitope of mouse LPAM-1 (alpha4beta7), which is a heterodimer of the the 154kDa alpha4 subunit and the 130kDa beta7 subunit. In mouse, the beta7 subunit is found on the majority of mature lymphocytes and a small population of thymocytes and bone marrow cells. LPAM-1 is an integrin involved in the transendothelial migration of lymphocytes across high endothelial venules (HEV), and is know to bind several ligands including MAdCAM-1, VCAM-1 and fibronectin. Binding of the DATK32 monoclonal antibody has been shown to induce aggregation of the CD8+ T cell lymphoma TK1, and block LPAM-1-mediated cell adhesion.Applications Reported: This DATK32 (DATK-32) antibody has been reported for use in flow cytometric analysis. (Please use Functional Grade purified DATK32 (DATK-32), cat. 16-5887, in functional assays.).Applications Tested: This DATK32 (DATK-32) antibody has been tested tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|Integrin alpha 4 beta 7 (LPAM-1)|
|PBS with 0.09% sodium azide; pH 7.2|
|ITGB7, Gut homing receptor beta subunit, Integrin alpha-4/beta-7, Peyer patches-specific homing receptor LPAM-1, Itga4|
|Flow Cytometry, Functional Assay|
|IgG2a, kappa, kappa|
|Store at 2-8°C.|
For Research Use Only.